Anti-Hemopexin Antibody [ARC1444] (A305314)

Name: Anti-Hemopexin Antibody [ARC1444]
Brand: Antibodies.com
SKU: A305314
Price: 470.0 USD
Availability: InStock

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$470

Rabbit monoclonal [ARC1444] antibody to Hemopexin for WB, IHC and ICC/IF.

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Delivery

Freight Charges

$40 to United States

Lead Time

5-8 Business Days

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Specifications

Name

Anti-Hemopexin Antibody [ARC1444]

Description

Rabbit monoclonal [ARC1444] antibody to Hemopexin.

Applications

WB, IHC, ICC/IF

Dilutions

WB: 1:500-1:2,000, IHC: 1:50-1:200, ICC/IF: 1:50-1:200

Reactivity

Human, Mouse, Rat

Immunogen

A synthetic peptide corresponding to a sequence within amino acids 363-462 of human Hemopexin (HPX) (P02790).

Sequence

AFICPGSSRLHIMAGRRLWWLDLKSGAQATWTELPWPHEKVDGALCMEKSLGPNSCSANGPGLYLIHGPNLYCYSDVEKLNAAKALPQPQNVTSLLGCTH

Host

Rabbit

Clonality

Monoclonal

Clone ID

ARC1444

Isotype

IgG

Conjugate

Unconjugated

Purification

Affinity purification.

Molecular Weight

70 kDa

Product Form

Liquid

Formulation

Supplied in Phosphate Buffered Saline, pH 7.3, with 50% Glycerol, 0.05% BSA, and 0.02% Sodium Azide.

Storage

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

Synonyms

Beta 1B glycoprotein , Beta-1B-glycoprotein, FLJ56652, HEMO, HEMO_HUMAN, Hpx, HX

Isotype Controls

Suitable Secondaries

See all Anti-Rabbit IgG Secondaries →

Disclaimer

This product is for research use only. It is not intended for diagnostic or therapeutic use.

Scientific Validation Data (6)

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Figure 1: Western Blot - Anti-Hemopexin Antibody [ARC1444] (A305314)

Western blot analysis of extracts of Mouse lung, using Anti-Hemopexin Antibody [ARC1444] (A305314) at 1:1,000 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 2min.

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Figure 2: Western Blot - Anti-Hemopexin Antibody [ARC1444] (A305314)

Western blot analysis of extracts of Human serum, using Anti-Hemopexin Antibody [ARC1444] (A305314) at 1:5,000 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 1s.

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Figure 3: Immunohistochemistry - Anti-Hemopexin Antibody [ARC1444] (A305314)

Immunohistochemistry analysis of paraffin-embedded rat liver using Anti-Hemopexin Antibody [ARC1444] (A305314) at a dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

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Figure 4: Immunofluorescence - Anti-Hemopexin Antibody [ARC1444] (A305314)

Immunofluorescence analysis of rat liver using Anti-Hemopexin Antibody [ARC1444] (A305314) at a dilution of 1:100 (40x lens). DAPI was used to stain the cell nuclei (blue).