Anti-Hes1 Antibody [ARC0513] (A305844)

Name: Anti-Hes1 Antibody [ARC0513]
Brand: Antibodies.com
SKU: A305844
Price: 470.0 USD
Availability: InStock

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$470

Rabbit monoclonal [ARC0513] antibody to Hes1 for WB, IHC and IP.

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Delivery

Freight Charges

$40 to United States

Lead Time

5-8 Business Days

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Neuroepithelial Marker Radial Glia Marker

Specifications

Name

Anti-Hes1 Antibody [ARC0513]

Description

Rabbit monoclonal [ARC0513] antibody to Hes1.

Applications

WB, IHC, IP

Dilutions

WB: 1:500-1:2,000, IHC: 1:50-1:200, IP: 1:100-1:500

Reactivity

Mouse, Rat

Immunogen

A synthetic peptide corresponding to a sequence within amino acids 181-280 of human HES1 (Q14469).

Sequence

FAPPPPLVPIPGGAAPPPGGAPCKLGSQAGEAAKVFGGFQVVPAPDGQFAFLIPNGAFAHSGPVIPVYTSNSGTSVGPNAVSPSSGPSLTADSMWRPWRN

Host

Rabbit

Clonality

Monoclonal

Clone ID

ARC0513

Isotype

IgG

Conjugate

Unconjugated

Purification

Affinity purification.

Molecular Weight

30 kDa

Product Form

Liquid

Formulation

Supplied in Phosphate Buffered Saline, pH 7.3, with 50% Glycerol, 0.05% BSA, and 0.02% Sodium Azide.

Storage

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

Synonyms

bHLHb39, C-HAIRY1, c-hairy1A, Class B basic helix-loop-helix protein 39, FLJ20408, Hairy and enhancer of split 1, Hairy and enhancer of split 1 (Drosophila), Hairy homolog, Hairy homolog (Drosophila), Hairy like, Hairy, Drosophila, homolog of, Hairy-like protein, Hairy/enhancer of split, Drosophila, homolog of, 1, HAIRY1, HES-1, Hes1 hairy and enhancer of split 1 (Drosophila), HES1_HUMAN, HHL, HL, HRY, MGC129109, OTTHUMP00000209031, RHL, Transcription factor HES-1

Isotype Controls

Suitable Secondaries

See all Anti-Rabbit IgG Secondaries →

Disclaimer

This product is for research use only. It is not intended for diagnostic or therapeutic use.

Scientific Validation Data (4)

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Figure 1: Western Blot - Anti-Hes1 Antibody [ARC0513] (A305844)

Western blot analysis of extracts of various cell lines, using Anti-Hes1 Antibody [ARC0513] (A305844) at 1:1,000 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 3min.

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Figure 2: Immunohistochemistry - Anti-Hes1 Antibody [ARC0513] (A305844)

Immunohistochemistry analysis of paraffin-embedded rat brain using Anti-Hes1 Antibody [ARC0513] (A305844) at a dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.

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Figure 3: Immunohistochemistry - Anti-Hes1 Antibody [ARC0513] (A305844)

Immunohistochemistry analysis of paraffin-embedded mouse spinal cord using Anti-Hes1 Antibody [ARC0513] (A305844) at a dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.

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Figure 4: Western Blot - Anti-Hes1 Antibody [ARC0513] (A305844)

Immunoprecipitation analysis of 600µg extracts of rat testis cells using 3µg of Anti-Hes1 Antibody [ARC0513] (A305844). This Western blot was performed on the immunoprecipitate using Anti-Hes1 Antibody [ARC0513] (A305844) at a dilution of 1:500.

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