Anti-Hexokinase II Antibody [ARC52060] (A307429)

Name: Anti-Hexokinase II Antibody [ARC52060]
Brand: Antibodies.com
SKU: A307429

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This product is discontinued

Anti-Hexokinase II Antibody [ARC52060] (A307429) has been discontinued and is no longer available.

View all Anti-Hexokinase II Antibodies.

Specifications

Name

Anti-Hexokinase II Antibody [ARC52060]

Description

Rabbit monoclonal [ARC52060] antibody to Hexokinase II.

Applications

WB, ICC/IF

Dilutions

WB: 1:1,000-1:5,000, ICC/IF: 1:50-1:200

Reactivity

Human, Mouse, Rat

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 1-120 of human Hexokinase II (NP_000180.2).

Sequence

MIASHLLAYFFTELNHDQVQKVDQYLYHMRLSDETLLEISKRFRKEMEKGLGATTHPTAAVKMLPTFVRSTPDGTEHGEFLALDLGGTNFRVLWVKVTDNGLQKVEMENQIYAIPEDIMR

Host

Rabbit

Clonality

Monoclonal

Clone ID

ARC52060

Isotype

IgG

Conjugate

Unconjugated

Purification

Affinity purification.

Molecular Weight

102 kDa

Product Form

Liquid

Formulation

Supplied in Phosphate Buffered Saline, pH 7.3, with 50% Glycerol, 0.05% BSA, and 0.05% Proclin 300.

Storage

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

Synonyms

DKFZp686M1669, Hexokinase 2, Hexokinase 2 muscle, Hexokinase type II, Hexokinase-2, HK 2, HK II, HK2, HKII, HxK 2, HxK2, HXK2_HUMAN, Muscle form hexokinase

Isotype Controls

Suitable Secondaries

See all Anti-Rabbit IgG Secondaries →

Disclaimer

This product is for research use only. It is not intended for diagnostic or therapeutic use.

Scientific Validation Data (3)

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Figure 1: Western Blot - Anti-Hexokinase II Antibody [ARC52060] (A307429)

Western blot analysis of extracts of various lysates, using Anti-Hexokinase II Antibody [ARC52060] (A307429) at 1:1,000 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 1s.

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Figure 2: Western Blot - Anti-Hexokinase II Antibody [ARC52060] (A307429)

Western blot analysis of extracts from wild type(WT) and Hexokinase II Rabbit mAb knockout (KO) HeLa cells, using Anti-Hexokinase II Antibody [ARC52060] (A307429) at 1:1,000 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 1s.

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Figure 3: Immunofluorescence - Anti-Hexokinase II Antibody [ARC52060] (A307429)

Immunofluorescence analysis of NIH/3T3 cells using Anti-Hexokinase II Antibody [ARC52060] (A307429) at a dilution of 1:50 (40x lens). DAPI was used to stain the cell nuclei (blue).

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