Anti-HINT1 Antibody [ARC2853] (A306842)

Name: Anti-HINT1 Antibody [ARC2853]
Brand: Antibodies.com
SKU: A306842
Price: 470.0 USD
Availability: InStock

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$470

Rabbit monoclonal [ARC2853] antibody to HINT1 for WB, IHC and ICC/IF.

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Delivery

Freight Charges

$40 to United States

Lead Time

5-8 Business Days

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+1 (314) 370-6046

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Specifications

Name

Anti-HINT1 Antibody [ARC2853]

Description

Rabbit monoclonal [ARC2853] antibody to HINT1.

Applications

WB, IHC, ICC/IF

Dilutions

WB: 1:500-1:1,000, IHC: 1:50-1:200, ICC/IF: 1:50-1:200

Reactivity

Human, Mouse, Rat

Immunogen

A synthetic peptide corresponding to a sequence within amino acids 1-100 of human HINT1 (P49773).

Sequence

MADEIAKAQVARPGGDTIFGKIIRKEIPAKIIFEDDRCLAFHDISPQAPTHFLVIPKKHISQISVAEDDDESLLGHLMIVGKKCAADLGLNKGYRMVVNE

Host

Rabbit

Clonality

Monoclonal

Clone ID

ARC2853

Isotype

IgG

Conjugate

Unconjugated

Purification

Affinity purification.

Molecular Weight

14 kDa

Product Form

Liquid

Formulation

Supplied in Phosphate Buffered Saline, pH 7.3, with 50% Glycerol, 0.05% BSA, and 0.02% Sodium Azide.

Storage

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

Synonyms

Adenosine 5' monophosphoramidase, Adenosine 5''-monophosphoramidase, HINT 1, HINT1_HUMAN, Histidine triad nucleotide binding protein 1, Histidine triad nucleotide-binding protein 1, PKCI 1, PKCI-1, PKCI1, PRKCNH1, Protein kinase C inhibitor 1, Protein kinase C interacting protein 1, Protein kinase C-interacting protein 1

Isotype Controls

Suitable Secondaries

See all Anti-Rabbit IgG Secondaries →

Disclaimer

This product is for research use only. It is not intended for diagnostic or therapeutic use.

Scientific Validation Data (9)

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Figure 1: Western Blot - Anti-HINT1 Antibody [ARC2853] (A306842)

Western blot analysis of extracts of various cell lines, using Anti-HINT1 Antibody [ARC2853] (A306842) at 1:1,000 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 3s.

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Figure 2: Western Blot - Anti-HINT1 Antibody [ARC2853] (A306842)

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Figure 3: Immunohistochemistry - Anti-HINT1 Antibody [ARC2853] (A306842)

Immunohistochemistry analysis of paraffin-embedded human breast cancer tissue using Anti-HINT1 Antibody [ARC2853] (A306842) at a dilution of 1:50 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.

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Figure 4: Immunohistochemistry - Anti-HINT1 Antibody [ARC2853] (A306842)

Immunohistochemistry analysis of paraffin-embedded human colon carcinoma tissue using Anti-HINT1 Antibody [ARC2853] (A306842) at a dilution of 1:50 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.