Anti-His Tag Antibody [6HIS/3550] (A250872)

Mouse monoclonal [6HIS/3550] antibody to His Tag for WB, Flow Cytometry, IF and IP.

Alternative Formats and ConjugatesAlternative Formats

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Name
Anti-His Tag Antibody [6HIS/3550]
Description
Mouse monoclonal [6HIS/3550] antibody to His Tag.
Specificity
This antibody recognizes both the free and protein-conjugated (either soluble or cell bound) form of hexa-histidine. This antibody is highly specific to hexa-histidine and shows no cross-reaction with other structurally related compounds.
Applications
WB, Flow Cytometry, IF, IP
Dilutions
WB: 1-2 µg/ml, Flow Cytometry: 0.5-1 µg/million cells, IF: 0.5-1 µg/ml, IP: 2-4 µg / 500 µg protein lysate
Immunogen
Recombinant hexa-histidine tagged human CD70 protein.
Host
Mouse
Clonality
Monoclonal
Clone ID
6HIS/3550
Isotype
IgG1
Light Chains
kappa
Conjugate

Unconjugated

Purification
Protein A/G chromatography.
Concentration
200 µg/ml
Molecular Weight
244Da
Product Form
Liquid
Formulation
Supplied in 10mM Phosphate Buffered Saline with 0.05% BSA and 0.05% Sodium Azide.
Storage
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
General Notes
This monoclonal antibody is also available in a different formulation without BSA and Sodium Azide - Anti-His Tag Antibody [6HIS/3550] - BSA and Azide free (A254052).
Synonyms
6 His epitope tag, 6X His tag, Hexa His tag, HHHHHH epitope tag, HHHHHH tag, Polyhistidine Tag
Disclaimer
This product is for research use only. It is not intended for diagnostic or therapeutic use.
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