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Recombinant Anti-His Tag Antibody [6HIS/6402R] (A251013)

SDS-PAGE - Anti-His Tag Antibody [6HIS/6402R] (A251012) - Antibodies.com Western Blot - Anti-His Tag Antibody [6HIS/6402R] (A251013) - Antibodies.com
Recombinant Antibody
$525
Product Datasheet

Recombinant rabbit monoclonal [6HIS/6402R] antibody to His Tag for WB, Flow Cytometry, IF and IP.

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$40 to United States
Lead Time
4-6 Business Days

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Mouse monoclonal [AMC0149] antibody to His Tag for WB and IP.
Name
Anti-His Tag Antibody [6HIS/6402R]
Description
Recombinant rabbit monoclonal [6HIS/6402R] antibody to His Tag.
Specificity
This antibody recognizes both the free and protein-conjugated (either soluble or cell bound) form of hexa-histidine. This antibody is highly specific to hexa-histidine and shows no cross-reaction with other structurally related compounds.
Applications
WB, Flow Cytometry, IF, IP
Dilutions
WB: 1-2 µg/ml, Flow Cytometry: 0.5-1 µg/million cells, IF: 0.5-1 µg/ml, IP: 2-4 µg / 500 µg protein lysate
Immunogen
Recombinant hexa-histidine tagged human CD70 protein.
Host
Rabbit
Clonality
Monoclonal
Clone ID
6HIS/6402R
Isotype
IgG
Light Chains
kappa
Conjugate

Unconjugated

Purification
Protein A/G chromatography.
Concentration
200 µg/ml
Molecular Weight
244Da
Product Form
Liquid
Formulation
Supplied in 10mM Phosphate Buffered Saline with 0.05% BSA and 0.05% Sodium Azide.
Storage
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
General Notes
This monoclonal antibody is also available in a different formulation without BSA and Sodium Azide - Anti-His Tag Antibody [6HIS/6402R] - BSA and Azide free (A254192).
Synonyms
6 His epitope tag, 6X His tag, Hexa His tag, HHHHHH epitope tag, HHHHHH tag, Polyhistidine Tag
Isotype Controls
Suitable Secondaries
Disclaimer
This product is for research use only. It is not intended for diagnostic or therapeutic use.
SDS-PAGE - Anti-His Tag Antibody [6HIS/6402R] (A251012) - Antibodies.comEnlarge Image
Figure 1: SDS-PAGE - Anti-His Tag Antibody [6HIS/6402R] (A251013)
SDS-PAGE analysis of Anti-His Tag Antibody [6HIS/6402R] under non-reduced and reduced conditions; showing intact IgG and intact heavy and light chains, respectively. SDS-PAGE analysis confirms the integrity and purity of the antibody.
Western Blot - Anti-His Tag Antibody [6HIS/6402R] (A251013) - Antibodies.comEnlarge Image
Figure 2: Western Blot - Anti-His Tag Antibody [6HIS/6402R] (A251013)
Western blot analysis of recombinant His tagged CELA3B protein using Anti-His Tag Antibody [6HIS/6402R].
Publishing research using Recombinant Anti-His Tag Antibody [6HIS/6402R] (A251013)? Please let us know so that we can list the citation on this page.

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