Figure 1: Western Blot - Anti-His Tag Antibody [ARC2696] (A309752)
Western blot analysis of extracts of His Protein, using Anti-His Tag Antibody [ARC2696] (A309752) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 10s.
Figure 2: Immunofluorescence - Anti-His Tag Antibody [ARC2696] (A309752)
Immunofluorescence analysis of 293T-SOST His(C) and 293T using Anti-His Tag Antibody [ARC2696] (A309752) at a dilution of 1:200 (40x lens). DAPI was used to stain the cell nuclei (blue).
Figure 3: Western Blot - Anti-His Tag Antibody [ARC2696] (A309752)
Immunoprecipitation analysis of 300µg extracts of 293T cells using 3µg of Anti-His Tag Antibody [ARC2696] (A309752). This Western blot was performed on the immunoprecipitate using Anti-His Tag Antibody [ARC2696] (A309752) at a dilution of 1:30000.
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