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Anti-His Tag Antibody

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Total Citations (68)
Mouse monoclonal (HIS.H8) antibody to His Tag from Antibodies.com (A85277).

   Please Note: This is exactly the same antibody that is marketed as:


 
   •  Abcam | ab18184
   •  Novus Biologicals | NBP2-31055
Name: Anti-His Tag Antibody
Description: Mouse monoclonal (HIS.H8) antibody to His Tag.
Specificity: Recognizes the epitope of 6x His-tags encoded by many commercially available vectors, regardless of the tag's location in the fusion protein sequences (i.e. reacts with N-terminal, C-terminal or internal 6x His-tags).
Applications: Dot, ELISA, IP, IS, WB
Immunogen: HHHHHH (6x His) synthetic peptide conjugated to KLH.
Host: Mouse
Clonality: Monoclonal
Clone: HIS.H8
Isotype: IgG2b
Conjugate: Unconjugated
Purification: Protein A affinity chromatography from mouse ascites fluid.
Concentration: 1 mg/ml
Product Form: Liquid
Formulation: Supplied in 10mM Phosphate Buffered Saline, pH 7.20, with 0.05% Sodium Azide.
Storage: Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Synonyms: 6 His epitope tag Antibody
6X His tag® Antibody
Hexa His tag Antibody
HHHHHH epitope tag Antibody
HHHHHH tag Antibody
Polyhistidine Tag Antibody
Immunofluorescence - Anti-His Tag Antibody from Antibodies.com (A85277)
Immunofluorescence (red) with Anti-His Tag Antibody on His-tag fusion protein in HEK293 cells. Left hand panel: untransfected control. Right hand panel: transfected.
Counterstained with DAPI (blue).
Western Blot - Anti-His Tag Antibody from Antibodies.com (A85277)
Standard ladder containing five different His-tagged proteins; untransfected (1), HEK293 cells transferred with His-tagged protein vector (2).
Western Blot - Anti-His Tag Antibody from Antibodies.com (A85277)
Comparison between Anti-His tag (HIS.H8 / EH158) mAb with 2 different vendor Abs, probed against a standard ladder containing five different His-tagged proteins. All Ab dilutions are 1:2000 (0.5ug/mL).
Western Blot - Anti-His Tag Antibody from Antibodies.com (A85277)
Western blot analysis of a 6x His-tagged protein was performed by loading 15µg of cell lysates from SF9 cells expressing an 11kD 6x-His tagged protein per well onto an SDS-PAGE gel. Proteins were transferred to a nitrocellulose membrane, and blocked in blocking buffer for 1 hour at room temperature. The membrane was probed with Anti-His Tag Antibody at a dilution of 1:500 overnight at 4 degrees celsius, washed in TBST, and probed with an Anti-Mouse IgG secondary antibody conjugated to a near-IR dye. Results were visualized using a near-IR imager.
Please Note: All batches are extensively validated and are guaranteed to work as specified, however, QC data is not necessarily from the current lot on sale.

   Please Note: These publications cite this antibody under different brand names & catalogue numbers.


Cites ab18184

Wang BD  et al. Alternative splicing promotes tumour aggressiveness and drug resistance in African American prostate cancer. Nat Commun 8:15921 (2017).

Cites ab18184

Tiede C  et al. Affimer proteins are versatile and renewable affinity reagents. Elife 6:N/A (2017).

Cites ab18184

Cloutier P  et al. R2TP/Prefoldin-like component RUVBL1/RUVBL2 directly interacts with ZNHIT2 to regulate assembly of U5 small nuclear ribonucleoprotein. Nat Commun 8:15615 (2017).

Cites ab18184

Chen X  et al. Interaction between granulin A and enolase 1 attenuates the migration and invasion of human hepatoma cells. Oncotarget 8:30305-30316 (2017).

Cites ab18184

Dickens D  et al. Modulation of LAT1 (SLC7A5) transporter activity and stability by membrane cholesterol. Sci Rep 7:43580 (2017).

Cites ab18184

El-Diwany R  et al. Extra-epitopic hepatitis C virus polymorphisms confer resistance to broadly neutralizing antibodies by modulating binding to scavenger receptor B1. PLoS Pathog 13:e1006235 (2017).

Cites ab18184

Zoltowska KM  et al. Dynamic presenilin 1 and synaptotagmin 1 interaction modulates exocytosis and amyloid ß production. Mol Neurodegener 12:15 (2017).

Cites ab18184

Wang B  et al. Brain-derived and glial cell line-derived neurotrophic factor fusion protein immobilization to laminin. Exp Ther Med 13:178-186 (2017).

Cites ab18184

Park CR  et al. The accessory proteins REEP5 and REEP6 refine CXCR1-mediated cellular responses and lung cancer progression. Sci Rep 6:39041 (2016).

Cites ab18184

Tillgren V  et al. The Tyrosine Sulfate Domain of Fibromodulin Binds Collagen and Enhances Fibril Formation. J Biol Chem 291:23744-23755 (2016).

Cites ab18184

Jenkinson EM  et al. Mutations in SNORD118 cause the cerebral microangiopathy leukoencephalopathy with calcifications and cysts. Nat Genet 48:1185-92 (2016).

Cites ab18184

Yi Z  et al. Affinity Purification of the Hepatitis C Virus Replicase Identifies Valosin-Containing Protein, a Member of the ATPases Associated with Diverse Cellular Activities Family, as an Active Virus Replication Modulator. J Virol 90:9953-9966 (2016).

Cites ab18184

Shimmon G  et al. Truncated Bovine Integrin Alpha-v/Beta-6 as a Universal Capture Ligand for FMD Diagnosis. PLoS One 11:e0160696 (2016).

Cites ab18184

Knyphausen P  et al. Insights into Lysine Deacetylation of Natively Folded Substrate Proteins by Sirtuins. J Biol Chem 291:14677-94 (2016).

Cites ab18184

Feng J  et al. Depletion of kinesin-12, a myosin-IIB-interacting protein, promotes migration of cortical astrocytes. J Cell Sci 129:2438-47 (2016).

Cites ab18184

Yu Z  et al. Lys29-linkage of ASK1 by Skp1-Cullin 1-Fbxo21 ubiquitin ligase complex is required for antiviral innate response. Elife 5:N/A (2016).

Cites ab18184

Conn KL  et al. Novel Role for Protein Inhibitor of Activated STAT 4 (PIAS4) in the Restriction of Herpes Simplex Virus 1 by the Cellular Intrinsic Antiviral Immune Response. J Virol 90:4807-26 (2016).

Cites ab18184

Jung B  et al. Pitchfork and Gprasp2 Target Smoothened to the Primary Cilium for Hedgehog Pathway Activation. PLoS One 11:e0149477 (2016).

Cites ab18184

Tung H  et al. Baculovirus IE2 Stimulates the Expression of Heat Shock Proteins in Insect and Mammalian Cells to Facilitate Its Proper Functioning. PLoS One 11:e0148578 (2016).

Cites ab18184

Booy EP  et al. RNA Helicase Associated with AU-rich Element (RHAU/DHX36) Interacts with the 3'-Tail of the Long Non-coding RNA BC200 (BCYRN1). J Biol Chem 291:5355-72 (2016).

Cites ab18184

Singh AB  et al. SREBP2 Activation Induces Hepatic Long-chain Acyl-CoA Synthetase 1 (ACSL1) Expression in Vivo and in Vitro through a Sterol Regulatory Element (SRE) Motif of the ACSL1 C-promoter. J Biol Chem 291:5373-84 (2016).

Cites ab18184

Sharma P  et al. Evolutionarily conserved intercalated disc protein Tmem65 regulates cardiac conduction and connexin 43 function. Nat Commun 6:8391 (2015).

Cites ab18184

Bauskar A  et al. Clusterin Seals the Ocular Surface Barrier in Mouse Dry Eye. PLoS One 10:e0138958 (2015).

Cites ab18184

Chalertpet K  et al. Human papillomavirus type 16 E7 oncoprotein mediates CCNA1 promoter methylation. Cancer Sci 106:1333-40 (2015).

Cites ab18184

Kamekura R  et al. Inflammation-induced desmoglein-2 ectodomain shedding compromises the mucosal barrier. Mol Biol Cell 26:3165-77 (2015).

Cites ab18184

Sloan E  et al. Analysis of the SUMO2 Proteome during HSV-1 Infection. PLoS Pathog 11:e1005059 (2015).

Cites ab18184

Aspalter IM  et al. Alk1 and Alk5 inhibition by Nrp1 controls vascular sprouting downstream of Notch. Nat Commun 6:7264 (2015).

Cites ab18184

Lee YL  et al. MAF1 represses CDKN1A through a Pol III-dependent mechanism. Elife 4:e06283 (2015).

Cites ab18184

LaFrance ME  et al. Identification of an epithelial cell receptor responsible for Clostridium difficile TcdB-induced cytotoxicity. Proc Natl Acad Sci U S A 112:7073-8 (2015).

Cites ab18184

Zehner M  et al. The translocon protein Sec61 mediates antigen transport from endosomes in the cytosol for cross-presentation to CD8(+) T cells. Immunity 42:850-63 (2015).

Cites ab18184

Singh S  et al. Upregulation of glycans containing 3' fucose in a subset of pancreatic cancers uncovered using fusion-tagged lectins. J Proteome Res 14:2594-605 (2015).

Cites ab18184

Wu K  et al. TREM-2 promotes macrophage survival and lung disease after respiratory viral infection. J Exp Med 212:681-97 (2015).

Cites ab18184

Tillgren V  et al. The novel small leucine-rich protein chondroadherin-like (CHADL) is expressed in cartilage and modulates chondrocyte differentiation. J Biol Chem 290:918-25 (2015).

Cites ab18184

Chen Q  et al. Whirlin and PDZ domain-containing 7 (PDZD7) proteins are both required to form the quaternary protein complex associated with Usher syndrome type 2. J Biol Chem 289:36070-88 (2014).

Cites ab18184

Ye J  et al. Reprogramming rat embryonic fibroblasts into induced pluripotent stem cells using transposon vectors and their chondrogenic differentiation in vitro. Mol Med Rep 11:989-94 (2015).

Cites ab18184

Lalonde J  et al. Store-operated calcium entry promotes the degradation of the transcription factor Sp4 in resting neurons. Sci Signal 7:ra51 (2014).

Cites ab18184

Liu X  et al. A peptide panel investigation reveals the acceptor specificity of O-GlcNAc transferase. FASEB J 28:3362-72 (2014).

Cites ab18184

Guo S  et al. Analysis of factors that affect FlgM-dependent type III secretion for protein purification with Salmonella enterica serovar Typhimurium. J Bacteriol 196:2333-47 (2014).

Cites ab18184

Lee WP  et al. Sentrin/SUMO specific proteases as novel tissue-selective modulators of vitamin D receptor-mediated signaling. PLoS One 9:e89506 (2014).

Cites ab18184

Booy EP  et al. The RNA helicase RHAU (DHX36) suppresses expression of the transcription factor PITX1. Nucleic Acids Res 42:3346-61 (2014).

Cites ab18184

Andresen CA  et al. Protein interaction screening for the ankyrin repeats and suppressor of cytokine signaling (SOCS) box (ASB) family identify Asb11 as a novel endoplasmic reticulum resident ubiquitin ligase. J Biol Chem 289:2043-54 (2014).

Cites ab18184

Meier M  et al. Binding of G-quadruplexes to the N-terminal recognition domain of the RNA helicase associated with AU-rich element (RHAU). J Biol Chem 288:35014-27 (2013).

Cites ab18184

Jung SM  et al. Smad6 inhibits non-canonical TGF-ß1 signalling by recruiting the deubiquitinase A20 to TRAF6. Nat Commun 4:2562 (2013).

Cites ab18184

Tartaglia LJ  et al. Molecular cloning, overexpression, and an efficient one-step purification of a5ß1 integrin. Protein Expr Purif 92:21-8 (2013).

Cites ab18184

Pace PE  et al. Hyperoxidized peroxiredoxin 2 interacts with the protein disulfide- isomerase ERp46. Biochem J 453:475-85 (2013).

Cites ab18184

Uthman S  et al. The amidation step of diphthamide biosynthesis in yeast requires DPH6, a gene identified through mining the DPH1-DPH5 interaction network. PLoS Genet 9:e1003334 (2013).

Cites ab18184

Che X  et al. ORF11 protein interacts with the ORF9 essential tegument protein in varicella-zoster virus infection. J Virol 87:5106-17 (2013).

Cites ab18184

Cloutier P  et al. A newly uncovered group of distantly related lysine methyltransferases preferentially interact with molecular chaperones to regulate their activity. PLoS Genet 9:e1003210 (2013).

Cites ab18184

Chen L  et al. N-terminal phosphorylation of phosphoprotein of vesicular stomatitis virus is required for preventing nucleoprotein from binding to cellular RNAs and for functional template formation. J Virol 87:3177-86 (2013).

Cites ab18184

Panter MS  et al. Dynamics of Major Histocompatibility Complex Class I Association with the Human Peptide-loading Complex. J Biol Chem 287:31172-84 (2012).

Cites ab18184

Goldfless SJ  et al. Direct and specific chemical control of eukaryotic translation with a synthetic RNA-protein interaction. Nucleic Acids Res 40:e64 (2012).

Cites ab18184

Brinkworth AJ  et al. Chlamydia trachomatis Slc1 is a type III secretion chaperone that enhances the translocation of its invasion effector substrate TARP. Mol Microbiol 82:131-44 (2011).

Cites ab18184

Booth DG  et al. A TACC3/ch-TOG/clathrin complex stabilises kinetochore fibres by inter-microtubule bridging. EMBO J 30:906-19 (2011).

Cites ab18184

Cojocaru M  et al. Transcription Factor IIS Cooperates with the E3 Ligase UBR5 to Ubiquitinate the CDK9 Subunit of the Positive Transcription Elongation Factor B. J Biol Chem 286:5012-22 (2011).

Cites ab18184

Sakurai K  et al. A role for human Dicer in pre-RISC loading of siRNAs. Nucleic Acids Res : (2010).

Cites ab18184

Rieger ME  et al. The embryonic transcription cofactor LBH is a direct target of the Wnt signaling pathway in epithelial development and in aggressive basal subtype breast cancers. Mol Cell Biol 30:4267-79 (2010).

Cites ab18184

Wenz C & Rüfer A Microchip CGE linked to immunoprecipitation as an alternative to Western blotting. Electrophoresis 30:4264-9 (2009).

Cites ab18184

Li W  et al. An eIF4E-binding protein regulates katanin protein levels in C. elegans embryos. J Cell Biol 187:33-42 (2009).

Cites ab18184

Denis H  et al. Functional connection between deimination and deacetylation of histones. Mol Cell Biol 29:4982-93 (2009).

Cites ab18184

Han C  et al. Human SCAMP5, a novel secretory carrier membrane protein, facilitates calcium-triggered cytokine secretion by interaction with SNARE machinery. J Immunol 182:2986-96 (2009).

Cites ab18184

Chattopadhyay S & Banerjee AK Phosphoprotein, P of human parainfluenza virus type 3 prevents self-association of RNA-dependent RNA polymerase, L. Virology 383:226-36 (2009).

Cites ab18184

Srinivasan A  et al. From the Cover: Quantitative biochemical rationale for differences in transmissibility of 1918 pandemic influenza A viruses. Proc Natl Acad Sci U S A 105:2800-2805 (2008).

Cites ab18184

Jacobsen LC  et al. The secretory leukocyte protease inhibitor (SLPI) and the secondary granule protein lactoferrin are synthesized in myelocytes, colocalize in subcellular fractions of neutrophils, and are coreleased by activated neutrophils. J Leukoc Biol 83:1155-64 (2008).

Cites ab18184

Boström P  et al. SNARE proteins mediate fusion between cytosolic lipid droplets and are implicated in insulin sensitivity. Nat Cell Biol 9:1286-93 (2007).

Cites ab18184

Chen M  et al. Interaction of vesicular stomatitis virus P and N proteins: identification of two overlapping domains at the N terminus of P that are involved in N0-P complex formation and encapsidation of viral genome RNA. J Virol 81:13478-85 (2007).

Cites ab18184

Kim WY  et al. ZEITLUPE is a circadian photoreceptor stabilized by GIGANTEA in blue light. Nature 449:356-60 (2007).

Cites ab18184

Hao Z  et al. Subcellular localization of CIAPIN1. J Histochem Cytochem 54:1437-44 (2006).

Cites ab18184

Hobbs RM  et al. Expression of activated MEK1 in differentiating epidermal cells is sufficient to generate hyperproliferative and inflammatory skin lesions. J Invest Dermatol 123:503-15 (2004).

Rating

Species

Application

Date

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Human

WB

11-Dec-2017

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Human

IP

16-Oct-2017

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Human

WB

13-Oct-2017

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Human

ICC/IF

13-Oct-2017

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Human

IP

25-Mar-2016

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Mouse

IHC-P

11-Feb-2016

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Human

WB

27-May-2015

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Escherichia coli

WB

15-Jan-2015

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Xenopus laevis

IHC

04-Aug-2014

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Human

WB

01-May-2013

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Human

WB

21-Feb-2013

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Human

IP

19-Dec-2012

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Human

WB

03-Dec-2012

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Human

WB

12-Jul-2012

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Human

ELISA

12-Jun-2012

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Rat

WB

13-Jul-2011

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Escherichia coli

WB

11-May-2011

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Human

ICC/IF

28-Dec-2009

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Human

WB

18-Dec-2009

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Escherichia coli

WB

17-Mar-2009

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Human

ICC/IF

30-Dec-2008

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Human

WB

17-Dec-2008

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Human

IP

17-Dec-2008

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Human

IP

01-Jul-2008

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Human

WB

08-May-2008

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Human

WB

02-Apr-2007


Description: Mouse monoclonal His tag antibody.
Application: WB, IF, IP
Host: Mouse
Isotype: IgG
Immunogen: A synthetic peptide of His tag.

£154 – £243

Description: Rat monoclonal (KT111) antibody to His tag.
Application: ELISA, WB
Host: Rat
Isotype: IgG2a
Immunogen: A protein antigen containing 6 his residues (HHHHHH).

£186 – £730

Description: Mouse monoclonal antibody to His Tag
Application: WB
Host: Mouse
Isotype: IgG1
Reactivity: Human

£267 – £388





Description: Mouse monoclonal (PHISSHG) antibody to His Tag
Host: Mouse
Isotype: IgG1
Product Form: Sterile Filtered solution.

£179 – £563





Please Note: Anti-His Tag Antibody is for research use only. It is not intended for diagnostic or therapeutic use.
Antibodies.com | A85277
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100µg £155
200µg £258

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