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Anti-Histone H1 (acetyl Lys25) Antibody (A94705)

Immunohistochemistry - Anti-Histone H1 (acetyl Lys25) Antibody (D0001) - Antibodies.com Western Blot - Anti-Histone H1 (acetyl Lys25) Antibody (D0001) - Antibodies.com
$185
Product Datasheet

Rabbit polyclonal antibody to Histone H1 (acetyl Lys25) for WB, IHC and ELISA.

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Freight Charges
$40 to United States
Lead Time
5-8 Business Days

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Name
Anti-Histone H1 (acetyl Lys25) Antibody
Description
Rabbit polyclonal antibody to Histone H1 (acetyl Lys25).
Specificity
This antibody detects endogenous levels of total Histone H1 protein only when acetylated at Lys25.
Applications
WB, IHC, ELISA
Dilutions
IHC: 1:50-1:100, IF: 1:100-1:500, ELISA: 1:1000
Reactivity
Human
Immunogen
Synthetic peptide derived from human Histone H1 around the acetylated site of Lys25 (amino acids 131-180).
Host
Rabbit
Clonality
Polyclonal
Isotype
IgG
Conjugate

Unconjugated

Purification
Purified from rabbit serum by antigen affinity chromatography using the immunizing acetylated peptide.
Molecular Weight
21kDa
Product Form
Liquid
Formulation
Supplied in Phosphate Buffered Saline (without Mg2+ and Ca2+), pH 7.4, with 150mM NaCl, 0.02% Sodium Azide, and 50% Glycerol.
Storage
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Synonyms
H1 histone family member O oocyte specific, H1FOO, H1FOO_HUMAN, Oocyte-specific histone H1, Oocyte-specific linker histone H1, osH1
Isotype Controls
Suitable Secondaries
Disclaimer
This product is for research use only. It is not intended for diagnostic or therapeutic use.
Immunohistochemistry - Anti-Histone H1 (acetyl Lys25) Antibody (D0001) - Antibodies.comEnlarge Image
Figure 1: Immunohistochemistry - Anti-Histone H1 (acetyl Lys25) Antibody (A94705)
Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using Anti-Histone H1 (acetyl Lys25) Antibody. The right hand panel represents a negative control, where the antibody was pre-incubated with the immunising peptide.
Western Blot - Anti-Histone H1 (acetyl Lys25) Antibody (D0001) - Antibodies.comEnlarge Image
Figure 2: Western Blot - Anti-Histone H1 (acetyl Lys25) Antibody (A94705)
Western blot analysis of lysates from COS7 cells, treated with TSA 400nM 24h using Anti-Histone H1 (acetyl Lys25) Antibody. The right hand lane represents a negative control, where the antibody is blocked by the immunising peptide.
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