Figure 1: Western Blot - Anti-Histone H2A.X Antibody [ARC0590] (A80673)
Western blot analysis of extracts of various cell lines, using Anti-Histone H2A.X Antibody [ARC0590] (A80673) at 1:1,000 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 60s.
Immunohistochemistry analysis of paraffin-embedded human liver cancer using Anti-Histone H2A.X Antibody [ARC0590] (A80673) at a dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.
Immunohistochemistry analysis of paraffin-embedded rat brain using Anti-Histone H2A.X Antibody [ARC0590] (A80673) at a dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.
Immunohistochemistry analysis of paraffin-embedded mouse brain using Anti-Histone H2A.X Antibody [ARC0590] (A80673) at a dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.
Chromatin immunoprecipitation (ChIP) analysis of extracts of HeLa cells, using Anti-Histone H2A.X Antibody [ARC0590] (A80673) and Rabbit IgG. The amount of immunoprecipitated DNA was checked by quantitative PCR. Histogram was constructed by the ratios of the immunoprecipitated DNA to the input.
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