Anti-Histone H3.3 (phospho Ser31) Antibody [ARC2788] (A308601)

Name: Anti-Histone H3.3 (phospho Ser31) Antibody [ARC2788]
Brand: Antibodies.com
SKU: A308601

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This product is discontinued

Anti-Histone H3.3 (phospho Ser31) Antibody [ARC2788] (A308601) has been discontinued and is no longer available.

View all Anti-Histone H3.3 Antibodies.

Specifications

Name

Anti-Histone H3.3 (phospho Ser31) Antibody [ARC2788]

Description

Rabbit monoclonal [ARC2788] antibody to Histone H3.3 (phospho Ser31).

Applications

WB, IHC

Dilutions

WB: 1:500-1:1,000, IHC: 1:50-1:200

Reactivity

Human, Mouse, Rat

Immunogen

A synthetic phosphorylated peptide around S31 of human Histone H3.3 (P84243).

Sequence

SAPST

Host

Rabbit

Clonality

Monoclonal

Clone ID

ARC2788

Isotype

IgG

Conjugate

Unconjugated

Purification

Affinity purification.

Molecular Weight

15 kDa

Product Form

Liquid

Formulation

Supplied in Phosphate Buffered Saline, pH 7.3, with 50% Glycerol, 0.05% BSA, and 0.02% Sodium Azide.

Storage

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

Synonyms

H3 histone family 3A, H3 histone family 3B, H3 histone, family 3B (H3.3B), H3.3, H3.3A, H3.3B, H33_HUMAN, H3F3, H3F3A, H3f3b, Histone H3.3Q, Histone H3.A, Histone H3.B, MGC87782, MGC87783

Isotype Controls

Suitable Secondaries

See all Anti-Rabbit IgG Secondaries →

Disclaimer

This product is for research use only. It is not intended for diagnostic or therapeutic use.

Scientific Validation Data (6)

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Figure 1: Western Blot - Anti-Histone H3.3 (phospho Ser31) Antibody [ARC2788] (A308601)

Western blot analysis of extracts of various cell lines, using Anti-Histone H3.3 (phospho Ser31) Antibody [ARC2788] (A308601) at 1:1,000 dilution. HeLa cells and C6 cells were treated by nocodazole (50 ng/ml) at 37°C for 20 hours. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 30s.

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Figure 2: Western Blot - Anti-Histone H3.3 (phospho Ser31) Antibody [ARC2788] (A308601)

Western blot analysis of extracts of NIH/3T3 cells, using Anti-Histone H3.3 (phospho Ser31) Antibody [ARC2788] (A308601) at 1:1,000 dilution. NIH/3T3 cells were treated by nocodazole (50 ng/ml) at 37°C for 20 hours. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 30s.

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Figure 3: Immunohistochemistry - Anti-Histone H3.3 (phospho Ser31) Antibody [ARC2788] (A308601)

Immunohistochemistry analysis of paraffin-embedded human esophageal cancer using Anti-Histone H3.3 (phospho Ser31) Antibody [ARC2788] (A308601) at a dilution of 1:50 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.

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Figure 4: Immunohistochemistry - Anti-Histone H3.3 (phospho Ser31) Antibody [ARC2788] (A308601)

Immunohistochemistry analysis of paraffin-embedded human liver cancer using Anti-Histone H3.3 (phospho Ser31) Antibody [ARC2788] (A308601) at a dilution of 1:50 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.

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