This antibody recognises Immunodeficiency Virus 1 gp41, gp120 and gp160 in western blot assays. ELISA based peptide mapping has confirmed high titer reactivity to HIV MN env peptide in the following regions: (6332)IEPLGVAPTKAKRRV, (6360)CTTTVPWNASWSNKS and moderate activity to multiple other regions on the env polypeptide. The viral envelope protein gp160 is cleaved into fragments gp120 and gp41. gp120 is an exterior envelope glycoprotein on the surface of the virus particle that binds to CD4 and chemokine receptors on macrophages and T-lymphocytes. gp41 is non-covalently bound to gp120 and aids the virus in entering the cell.
Applications
WB, ELISA
Dilutions
WB: 1:2,000
Reactivity
Viral
Immunogen
Recombinant HIV1 gp41 antigen.
Host
Goat
Clonality
Polyclonal
Isotype
IgG
Conjugate
Unconjugated
Concentration
1 mg/ml
Purity
>98%.
Product Form
Liquid
Formulation
Supplied in Isotonic Saline Solution, pH 6 - 7, with <0.1% Sodium Azide.
Storage
Shipped at ambient temperature. Upon delivery aliquot and store at -20°C. When thawed, aliquot the sample as needed. Short term (up to 4 weeks): store at 4°C. Long term: store at -20°C. Avoid freeze / thaw cycles. Storage in frost free freezers is not recommended.
Synonyms
Env, Glycoprotein 41, gp41, Human Immunodeficiency Virus 1, TM, Transmembrane protein
