Anti-HLA G Antibody [5A6G7] (A85877)

Anti-HLA G Antibody [5A6G7]

Mouse monoclonal [5A6G7] antibody to HLA G.

This antibody does not cross-react with the full-length HLA G1 isoform and can be used to distinguish secreted HLA G5 and HLA G6 from shedded HLA G1.

WB, IHC-P, IHC-Fr, ICC, ELISA, Flow Cytometry

Flow Cytometry: 1-4 µg/ml; Intracellular staining, IHC-Fr: 10 µg/ml; Positive Control: placenta, IHC-P: 10 µg/ml; Positive Control: placenta, ELISA: Positive Control: HeLa/HLA-G5 transfectants cell lysate, HeLa/HLA-G5 cell supernatant; negative control: HeLa cell lysate. This antibody has been tested as the capture antibody in a sandwich ELISA for analysis of soluble HLA-G in combination with antibody W6/32 (cat. no. 1B-422-C100), WB: Positive Control: JEG-3 cell lysate; Reducing conditions, 12% AA SDS-PAGE.

Human

C-terminal amino acid sequence (22-mer) of soluble HLA-G5 and HLA-G6 proteins coupled to ovalbumin.

Mouse

Monoclonal

5A6G7

IgG1

Protein A chromatography.

1 mg/ml

Liquid

Supplied in Phosphate Buffered Saline, pH 7.4, with 15 mM Sodium Azide.

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze/thaw cycles.

B2 microglobulin, DADB-15K14.8, HLA 6.0, HLA class I histocompatibility antigen alpha chain G, HLA class I histocompatibility antigen, alpha chain G, HLA class I molecule, HLA G antigen, HLA G histocompatibility antigen class I G, HLA G3, HLA-G, HLA-G histocompatibility antigen, class I, HLA60, HLAG, HLAG_HUMAN, Major histocompatibility complex class I G, MHC class I antigen, MHC class I antigen G, MHC G, T-cell A locus, TCA