Anti-HMGB1 Antibody [ARC0001] (A308594)

Name: Anti-HMGB1 Antibody [ARC0001]
Brand: Antibodies.com
SKU: A308594
Price: 470.0 USD
Availability: InStock

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$470

Rabbit monoclonal [ARC0001] antibody to HMGB1 for WB, IHC and ICC/IF.

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Freight Charges

$40 to United States

Lead Time

5-8 Business Days

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Knockout Validated Antibody

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Specifications

Name

Anti-HMGB1 Antibody [ARC0001]

Description

Rabbit monoclonal [ARC0001] antibody to HMGB1.

Applications

WB, IHC, ICC/IF

Dilutions

WB: 1:500-1:2,000, IHC: 1:50-1:200, ICC/IF: 1:50-1:200

Reactivity

Human, Mouse, Rat

Immunogen

A synthetic peptide corresponding to a sequence within amino acids 100-200 of human HMGB1 (P09429).

Sequence

SAFFLFCSEYRPKIKGEHPGLSIGDVAKKLGEMWNNTAADDKQPYEKKAAKLKEKYEKDIAAYRAKGKPDAAKKGVVKAEKSKKKKEEEEDEEDEEDEEEE

Host

Rabbit

Clonality

Monoclonal

Clone ID

ARC0001

Isotype

IgG

Conjugate

Unconjugated

Purification

Affinity purification.

Molecular Weight

25 kDa

Product Form

Liquid

Formulation

Supplied in Phosphate Buffered Saline, pH 7.3, with 50% Glycerol, 0.05% BSA, and 0.02% Sodium Azide.

Storage

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

Synonyms

Amphoterin, Chromosomal protein, nonhistone, HMG1, DKFZp686A04236, High mobility group 1, High mobility group box 1, High mobility group protein 1, High mobility group protein B1, high-mobility group (nonhistone chromosomal) protein 1, HMG-1, HMG1, HMG3, HMGB 1, HMGB1_HUMAN, NONHISTONE CHROMOSOMAL PROTEIN HMG1, SBP 1, Sulfoglucuronyl carbohydrate binding protein

Isotype Controls

Suitable Secondaries

See all Anti-Rabbit IgG Secondaries →

Disclaimer

This product is for research use only. It is not intended for diagnostic or therapeutic use.

Scientific Validation Data (8)

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Figure 1: Western Blot - Anti-HMGB1 Antibody [ARC0001] (A308594)

Western blot analysis of extracts of various cell lines, using Anti-HMGB1 Antibody [ARC0001] (A308594) at 1:1,000 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 5s.

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Figure 2: Western Blot - Anti-HMGB1 Antibody [ARC0001] (A308594)

Western blot analysis of extracts from wild type (WT) and HMGB1 knockout (KO) 293T cells, using Anti-HMGB1 Antibody [ARC0001] (A308594) at 1:1,000 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 5s.

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Figure 3: Western Blot - Anti-HMGB1 Antibody [ARC0001] (A308594)

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Figure 4: Immunohistochemistry - Anti-HMGB1 Antibody [ARC0001] (A308594)

Immunohistochemistry analysis of paraffin-embedded rat brain using Anti-HMGB1 Antibody [ARC0001] (A308594) at a dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.