Anti-HMGCR Antibody (A305714)

Name: Anti-HMGCR Antibody
Brand: Antibodies.com
SKU: A305714

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This product is discontinued

Anti-HMGCR Antibody (A305714) has been discontinued and is no longer available.

View all Anti-HMGCR Antibodies.

Specifications

Name

Anti-HMGCR Antibody

Description

Rabbit polyclonal antibody to HMGCR.

Applications

WB, ICC/IF

Dilutions

WB: 1:500-1:1,000, ICC/IF: 1:50-1:200

Reactivity

Human, Mouse, Rat

Immunogen

A synthetic peptide corresponding to a sequence within amino acids 700-800 of human HMGCR (NP_000850.1).

Sequence

EGRGKSVVCEAVIPAKVVREVLKTTTEAMIEVNINKNLVGSAMAGSIGGYNAHAANIVTAIYIACGQDAAQNVGSSNCITLMEASGPTNEDLYISCTMPSI

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Conjugate

Unconjugated

Purification

Affinity purification.

Product Form

Liquid

Formulation

Supplied in Phosphate Buffered Saline, pH 7.3, with 50% Glycerol and 0.05% Proclin 300.

Storage

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

Synonyms

3 hydroxy 3 methylglutaryl CoA reductase, 3 hydroxy 3 methylglutaryl Coenzyme A reductase, 3 hydroxymethylglutaryl CoA reductase, 3-hydroxy-3-methylglutaryl CoA reductase (NADPH), 3-hydroxy-3-methylglutaryl-coenzyme A reductase, 3H3M, HMDH_HUMAN, HMG CoA reductase, HMG CoAR, HMG-CoA reductase, Hydroxymethylglutaryl CoA reductase, LDLCQ3, MGC103269, Red

Isotype Controls

Suitable Secondaries

See all Anti-Rabbit IgG Secondaries →

Disclaimer

This product is for research use only. It is not intended for diagnostic or therapeutic use.

Scientific Validation Data (4)

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Figure 1: Western Blot - Anti-HMGCR Antibody (A305714)

Western blot analysis of various lysates, using Anti-HMGCR Antibody (A305714) at 1:1,000 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 30.

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Figure 2: Immunofluorescence - Anti-HMGCR Antibody (A305714)

Immunofluorescence analysis of A-549 cells using Anti-HMGCR Antibody (A305714) at a dilution of 1:200 (40x lens). DAPI was used to stain the cell nuclei (blue).

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Figure 3: Immunofluorescence - Anti-HMGCR Antibody (A305714)

Immunofluorescence analysis of HeLa cells using Anti-HMGCR Antibody (A305714) at a dilution of 1:200 (40x lens). DAPI was used to stain the cell nuclei (blue).

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Figure 4: Immunofluorescence - Anti-HMGCR Antibody (A305714)

Immunofluorescence analysis of NIH/3T3 cells using Anti-HMGCR Antibody (A305714) at a dilution of 1:200 (40x lens). DAPI was used to stain the cell nuclei (blue).

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