Anti-HMGCS2 Antibody (A90273)

Name: Anti-HMGCS2 Antibody
Brand: Antibodies.com
SKU: A90273
Price: 335.0 USD
Availability: InStock

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$335

Rabbit polyclonal antibody to HMGCS2 for WB, IHC and ICC/IF.

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Delivery

Freight Charges

$40 to United States

Lead Time

5-8 Business Days

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+1 (314) 370-6046

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Specifications

Name

Anti-HMGCS2 Antibody

Description

Rabbit polyclonal antibody to HMGCS2.

Applications

WB, IHC, ICC/IF

Dilutions

WB: 1:500-1:2,000, IHC: 1:50-1:100, ICC/IF: 1:50-1:100

Reactivity

Human, Mouse, Rat

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 269-508 of human HMGCS2 (NP_005509.1).

Sequence

CYTSYRKKIQNQWKQAGSDRPFTLDDLQYMIFHTPFCKMVQKSLARLMFNDFLSASSDTQTSLYKGLEAFGGLKLEDTYTNKDLDKALLKASQDMFDKKTKASLYLSTHNGNMYTSSLYGCLASLLSHHSAQELAGSRIGAFSYGSGLAASFFSFRVSQDAAPGSPLDKLVSSTSDLPKRLASRKCVSPEEFTEIMNQREQFYHKVNFSPPGDTNSLFPGTWYLERVDEQHRRKYARRPV

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Conjugate

Unconjugated

Purification

Affinity purification.

Molecular Weight

50 kDa

Product Form

Liquid

Formulation

Supplied in Phosphate Buffered Saline, pH 7.3, with 50% Glycerol and 0.01% Thiomersal.

Storage

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

Synonyms

3 hydroxy 3 methylglutaryl coenzyme A synthase, 3 hydroxy 3 methylglutaryl Coenzyme A synthase 2, 3 hydroxy 3 methylglutaryl Coenzyme A synthase 2 (mitochondrial), 3-hydroxy-3-methylglutaryl coenzyme A synthase, HMCS2_HUMAN, HMG CoA synthase, HMG-CoA synthase, HMGCS 2, Hydroxymethylglutaryl CoA synthase, Hydroxymethylglutaryl CoA synthase mitochondrial, Hydroxymethylglutaryl-CoA synthase, mitochondrial

Isotype Controls

Suitable Secondaries

See all Anti-Rabbit IgG Secondaries →

Disclaimer

This product is for research use only. It is not intended for diagnostic or therapeutic use.

Scientific Validation Data (6)

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Figure 1: Western Blot - Anti-HMGCS2 Antibody (A90273)

Western blot analysis of extracts of various cell lines, using Anti-HMGCS2 Antibody (A90273) at 1:1,000 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 30s.

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Figure 2: Immunohistochemistry - Anti-HMGCS2 Antibody (A90273)

Immunohistochemistry analysis of paraffin-embedded human thyroid cancer using Anti-HMGCS2 Antibody (A90273) at a dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.

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Figure 3: Immunohistochemistry - Anti-HMGCS2 Antibody (A90273)

Immunohistochemistry analysis of paraffin-embedded human colon carcinoma tissue using Anti-HMGCS2 Antibody (A90273) at a dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.

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Figure 4: Immunofluorescence - Anti-HMGCS2 Antibody (A90273)

Immunofluorescence analysis of rat liver using Anti-HMGCS2 Antibody (A90273) at a dilution of 1:100 (40x lens). DAPI was used to stain the cell nuclei (blue).