Anti-HMT Antibody (A14029)

Name: Anti-HMT Antibody
Brand: Antibodies.com
SKU: A14029
Price: 335.0 USD
Availability: InStock

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$335

Rabbit polyclonal antibody to HMT for WB, ICC/IF and IP.

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Delivery

Freight Charges

$40 to United States

Lead Time

5-8 Business Days

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Telephone

+1 (314) 370-6046

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Specifications

Name

Anti-HMT Antibody

Description

Rabbit polyclonal antibody to HMT.

Applications

WB, ICC/IF, IP

Dilutions

WB: 1:500-1:2,000, ICC/IF: 1:50-1:100, IP: 1:50-1:200

Reactivity

Human, Mouse

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 1-292 of human HNMT (NP_008826.1).

Sequence

MASSMRSLFSDHGKYVESFRRFLNHSTEHQCMQEFMDKKLPGIIGRIGDTKSEIKILSIGGGAGEIDLQILSKVQAQYPGVCINNEVVEPSAEQIAKYKELVAKTSNLENVKFAWHKETSSEYQSRMLEKKELQKWDFIHMIQMLYYVKDIPATLKFFHSLLGTNAKMLIIVVSGSSGWDKLWKKYGSRFPQDDLCQYITSDDLTQMLDNLGLKYECYDLLSTMDISDCFIDGNENGDLLWDFLTETCNFNATAPPDLRAELGKDLQEPEFSAKKEGKVLFNNTLSFIVIEA

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Conjugate

Unconjugated

Purification

Affinity purification.

Molecular Weight

33 kDa

Product Form

Liquid

Formulation

Supplied in Phosphate Buffered Saline, pH 7.3, with 50% Glycerol and 0.02% Sodium Azide.

Storage

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

Synonyms

EC 2.1.1.8, Histamine N methyltransferase, Histamine N-methyltransferase, HNMT, HNMT S1, HNMT S2, HNMT_HUMAN

Isotype Controls

Suitable Secondaries

See all Anti-Rabbit IgG Secondaries →

Disclaimer

This product is for research use only. It is not intended for diagnostic or therapeutic use.

Scientific Validation Data (3)

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Figure 1: Western Blot - Anti-HMT Antibody (A14029)

Western blot analysis of extracts of various cell lines, using Anti-HMT Antibody (A14029) at 1:1,000 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 90s.

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Figure 2: Immunofluorescence - Anti-HMT Antibody (A14029)

Immunofluorescence analysis of U2OS cells using Anti-HMT Antibody (A14029). DAPI was used to stain the cell nuclei (blue).

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Figure 3: Western Blot - Anti-HMT Antibody (A14029)

Immunoprecipitation analysis of 200µg extracts of HT-29 cells using 1µg of Anti-HMT Antibody (A14029). This Western blot was performed on the immunoprecipitate using Anti-HMT Antibody (A14029) at a dilution of 1:1000. Detection was with a ECL Enhanced Kit. Exposure time: 10s.

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