Anti-HNF-4-alpha Antibody [ARC2794] (A307694)

Name: Anti-HNF-4-alpha Antibody [ARC2794]
Brand: Antibodies.com
SKU: A307694
Price: 470.0 USD
Availability: InStock

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$470

Rabbit monoclonal [ARC2794] antibody to HNF-4-alpha for WB, IHC, IP and ChIP.

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Delivery

Freight Charges

$40 to United States

Lead Time

5-8 Business Days

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Specifications

Name

Anti-HNF-4-alpha Antibody [ARC2794]

Description

Rabbit monoclonal [ARC2794] antibody to HNF-4-alpha.

Applications

WB, IHC, IP, ChIP

Dilutions

WB: 1:500-1:1,000, IHC: 1:50-1:200, ChIP: 1:50-1:200

Reactivity

Human, Mouse, Rat

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 1-200 of mouse Recombinant protein of Mouse HNF-4-alpha (P49698).

Sequence

MRLSKTLAGMDMADYSAALDPAYTTLEFENVQVLTMGNDTSPSEGANLNSSNSLGVSALCAICGDRATGKHYGASSCDGCKGFFRRSVRKNHMYSCRFSRQCVVDKDKRNQCRYCRLKKCFRAGMKKEAVQNERDRISTRRSSYEDSSLPSINALLQAEVLSQQITSPISGINGDIRAKKIANITDVCESMKEQLLVLVE

Host

Rabbit

Clonality

Monoclonal

Clone ID

ARC2794

Isotype

IgG

Conjugate

Unconjugated

Purification

Affinity purification.

Molecular Weight

53 kDa

Product Form

Liquid

Formulation

Supplied in Phosphate Buffered Saline, pH 7.3, with 50% Glycerol, 0.05% BSA, and 0.02% Sodium Azide.

Storage

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

Synonyms

FLJ39654, FRTS4, Hepatic nuclear factor 4 alpha, Hepatocyte nuclear factor 4, Hepatocyte nuclear factor 4 alpha, Hepatocyte nuclear factor 4-alpha, HNF 4, HNF 4 alpha, HNF4, HNF4A, HNF4a7, HNF4a8, HNF4a9, Hnf4alpha, HNF4alpha10/11/12, HNF4A_HUMAN, MODY, MODY 1, MODY1, NR2A1, NR2A21, Nuclear receptor subfamily 2 group A member 1, OTTHUMP00000031060, OTTHUMP00000031062, TCF, TCF 14, TCF-14, TCF14, Tcf4, Transcription factor 14, Transcription factor 14, hepatic nuclear factor, Transcription factor HNF 4, Transcription factor HNF-4, Transcription factor HNF4

Isotype Controls

Suitable Secondaries

See all Anti-Rabbit IgG Secondaries →

Disclaimer

This product is for research use only. It is not intended for diagnostic or therapeutic use.

Scientific Validation Data (5)

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Figure 1: Western Blot - Anti-HNF-4-alpha Antibody [ARC2794] (A307694)

Western blot analysis of extracts of various cell lines, using Anti-HNF-4-alpha Antibody [ARC2794] (A307694) at 1:1,000 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 3s.

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Figure 2: Immunohistochemistry - Anti-HNF-4-alpha Antibody [ARC2794] (A307694)

Immunohistochemistry analysis of paraffin-embedded human colon carcinoma tissue using Anti-HNF-4-alpha Antibody [ARC2794] (A307694) at a dilution of 1:100 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.

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Figure 3: Immunohistochemistry - Anti-HNF-4-alpha Antibody [ARC2794] (A307694)

Immunohistochemistry analysis of paraffin-embedded human liver cancer using Anti-HNF-4-alpha Antibody [ARC2794] (A307694) at a dilution of 1:100 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.

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Figure 4: Immunohistochemistry - Anti-HNF-4-alpha Antibody [ARC2794] (A307694)

Immunohistochemistry analysis of paraffin-embedded human small intestine using Anti-HNF-4-alpha Antibody [ARC2794] (A307694) at a dilution of 1:100 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.