Anti-HSD3B1 Antibody [ARC2437] (A308449)

Name: Anti-HSD3B1 Antibody [ARC2437]
Brand: Antibodies.com
SKU: A308449
Price: 470.0 USD
Availability: InStock

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$470

Rabbit monoclonal [ARC2437] antibody to HSD3B1 for WB and IHC.

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Delivery

Freight Charges

$40 to United States

Lead Time

5-8 Business Days

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Specifications

Name

Anti-HSD3B1 Antibody [ARC2437]

Description

Rabbit monoclonal [ARC2437] antibody to HSD3B1.

Applications

WB, IHC

Dilutions

WB: 1:500-1:1,000, IHC: 1:50-1:200

Reactivity

Human, Mouse, Rat

Immunogen

A synthetic peptide corresponding to a sequence within amino acids 250-350 of human HSD3B1 (P14060).

Sequence

RGQFYYISDDTPHQSYDNLNYTLSKEFGLRLDSRWSFPLSLMYWIGFLLEIVSFLLRPIYTYRPPFNRHIVTLSNSVFTFSYKKAQRDLAYKPLYSWEEAK

Host

Rabbit

Clonality

Monoclonal

Clone ID

ARC2437

Isotype

IgG

Conjugate

Unconjugated

Purification

Affinity purification.

Molecular Weight

42 kDa

Product Form

Liquid

Formulation

Supplied in Phosphate Buffered Saline, pH 7.3, with 50% Glycerol, 0.05% BSA, and 0.02% Sodium Azide.

Storage

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

Synonyms

3 beta and steroid delta isomerase 1, 3 beta and steroid hydroxy delta 5 steroid dehydrogenase, 3 beta HSD I, 3 beta-hydroxysteroid dehydrogenase/Delta 5-->4-isomerase type I, 3-beta-HSD I, 3-beta-hydroxy-5-ene steroid dehydrogenase, 3BETAHSD, 3BH, 3BHS1_HUMAN, 3BHSD, Delta-5-3-ketosteroid isomerase, HGNC:5217, HSD3B, HSDB3, HSDB3A, Hydroxy delta 5 steroid dehydrogenase, Hydroxy delta 5 steroid dehydrogenase 3 beta and steroid delta isomerase 1, Progesterone reductase, SDR11E1, Steroid Delta-isomerase, trophoblast antigen, Trophoblast antigen FDO161G

Isotype Controls

Suitable Secondaries

See all Anti-Rabbit IgG Secondaries →

Disclaimer

This product is for research use only. It is not intended for diagnostic or therapeutic use.

Scientific Validation Data (3)

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Figure 1: Western Blot - Anti-HSD3B1 Antibody [ARC2437] (A308449)

Western blot analysis of extracts of various cell lines, using Anti-HSD3B1 Antibody [ARC2437] (A308449) at 1:1,000 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 90s.

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Figure 2: Immunohistochemistry - Anti-HSD3B1 Antibody [ARC2437] (A308449)

Immunohistochemistry analysis of paraffin-embedded rat ovary using Anti-HSD3B1 Antibody [ARC2437] (A308449) at a dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

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Figure 3: Immunohistochemistry - Anti-HSD3B1 Antibody [ARC2437] (A308449)

Immunohistochemistry analysis of paraffin-embedded human placenta using Anti-HSD3B1 Antibody [ARC2437] (A308449) at a dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

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