Recombinant Anti-HSP27 Antibody [rHSPB1/7298] (A315101)

Anti-HSP27 Antibody [rHSPB1/7298]

Recombinant mouse monoclonal [rHSPB1/7298] antibody to HSP27.

HSP27, also referred to as the Estrogen-Regulated 24K protein and HSP28, is one of several small heat shock proteins produced by all organisms studied. HSP27 synthesis is induced by elevated temperature, as well as by estrogen in hormone responsive cells. Interestingly, human HSP27 also shares greater than 50% homology with low molecular weight Drosophila HSPs and mammalian -crystalline lens protein. Because of the estrogen responsive nature of HSP27, this protein has been studied extensively in human estrogen responsive tissues such as cervix, endometrium and breast tissue. Therefore, HSP27 may be useful in classifying various hormone sensitive tumors.

MCF-7 or HeLa cells. ~50% of breast carcinomas are positive for hsp27 especially those that are ER and/or PgR positive

IHC: 1-2 µg/ml

Human

Recombinant human full-length HSP27 protein.

Mouse

Monoclonal

rHSPB1/7298

IgG1

Unconjugated

Protein A/G chromatography.

200 µg/ml

27 kDa

Liquid

Supplied in 10mM Phosphate Buffered Saline with 0.05% BSA and 0.05% Sodium Azide.

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

28 kDa heat shock protein, CMT2F, DKFZp586P1322, epididymis secretory protein Li 102, Estrogen regulated 24 kDa protein, Estrogen-regulated 24 kDa protein, Heat shock 25kDa protein 1, Heat shock 27 kDa protein, Heat shock 27kD protein 1, Heat shock 27kDa protein, Heat shock 27kDa protein 1, Heat shock 28kDa protein 1, Heat Shock Protein 27, Heat shock protein beta 1, Heat shock protein beta-1, heat shock protein family B (small) member 1, HEL-S-102, HMN2B, HS.76067, Hsp 25, HSP 27, Hsp 28, Hsp B1, Hsp25, Hsp28, HspB1, HSPB1_HUMAN, SRP27, Stress responsive protein 27, Stress-responsive protein 27