Anti-HSP60 Antibody (A305092)

Name: Anti-HSP60 Antibody
Brand: Antibodies.com
SKU: A305092
Price: 455.0 USD
Availability: InStock

Enlarge Image

$455

Rabbit polyclonal antibody to HSP60 for WB, IHC, IP and ELISA.

100% Guarantee

Price Match Guarantee

Delivery

Freight Charges

$40 to United States

Lead Time

5-8 Business Days

Contact Us

Telephone

+1 (314) 370-6046

orders@antibodies.com

Guarantees

100% Quality Guarantee

Price Match Guarantee

Tags:

Mitochondrial Loading Control Mitochondrial Marker

Specifications

Name

Anti-HSP60 Antibody

Description

Rabbit polyclonal antibody to HSP60.

Applications

WB, IHC, IP, ELISA

Dilutions

WB: 1:1,000, ICC/IF: 1:100

Reactivity

Human, Mouse, Rat, Bovine, Canine, Chicken, Rabbit, Hamster

Immunogen

Human HSP60 produced through recombinant DNA methods in E.coli.

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Conjugate

Unconjugated

Purification

Protein A purification.

Concentration

1 mg/ml

Molecular Weight

~60 kDa

Product Form

Liquid

Formulation

Supplied in Phosphate Buffered Saline with 50% Glycerol and 0.09% Sodium Azide.

Storage

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

Synonyms

60 kDa chaperonin, 60 kDa heat shock protein, mitochondrial, CH60_HUMAN, Chaperonin, Chaperonin 60, Chaperonin, 60-KD, CPN60, fa04a05, GROEL, Heat shock 60kDa protein 1, heat shock 60kDa protein 1 (chaperonin), Heat shock protein 1 (chaperonin), Heat shock protein 60, Heat shock protein 65, heat shock protein family D (Hsp60) member 1, HLD4, Hsp 60, HSP 65, HSP-60, HSP65, HSPD1, HuCHA60, Mitochondrial matrix protein P1, P60 lymphocyte protein, short heat shock protein 60 Hsp60s1, SPG13

Isotype Controls

Suitable Secondaries

See all Anti-Rabbit IgG Secondaries →

Disclaimer

This product is for research use only. It is not intended for diagnostic or therapeutic use.

Scientific Validation Data (4)

Enlarge Image

Figure 1: Immunofluorescence - Anti-HSP60 Antibody (A305092)

Immunocytochemistry/Immunofluorescence analysis of human heat shocked cervical cancer cell line (HeLa), fixed in 2% formaldehyde for 20 minutes at room temperature, using Anti-HSP60 Antibody (A305092), at 1:100 for 12 hours at 4°C. The secondary antibody used was APC Goat Anti-Rabbit (red) at 1:200 for 2 hours at room temperature. Counterstain: DAPI (blue) nuclear stain at 1:40000 for 2 hours at room temperature. Localization: Mitochondrion matrix. Magnification: 20x.(A) DAPI (blue) nuclear stain. (B) Anti-Hsp60 Antibody. (C) Composite. Heat Shocked at 42°C for 1h.

Enlarge Image

Figure 2: Western Blot - Anti-HSP60 Antibody (A305092)

Western blot analysis of Human, Dog, mouse SKBR3, MDCK, and MEF cell line lysates showing detection of HSP60 protein using Anti-HSP60 Antibody (A305092) at 1:1,000. Lane 1: Recom. human Hsp60 (100ng), Lane2, 3 and 4: SKBR3 lysate (human), MDCK lysate (dog) and MEF lysate (mouse) (Al at 7.5ug).

Enlarge Image

Figure 3: Immunohistochemistry - Anti-HSP60 Antibody (A305092)

Immunohistochemistry analysis of human adrenal, fixed in formalin and paraffin-embedded. The Primary Antibody used was Anti-HSP60 Antibody (A305092).

Enlarge Image

Figure 4: Immunofluorescence - Anti-HSP60 Antibody (A305092)

Immunocytochemistry/Immunofluorescence analysis of human heat shocked cervical cancer cell line (HeLa), fixed in 2% formaldehyde for 20 minutes at room temperature, using Anti-HSP60 Antibody (A305092), at 1:100 for 12 hours at 4°C. The secondary antibody used was FITC Goat Anti-Rabbit (green) at 1:200 for 2 hours at room temperature. Counterstain: DAPI (blue) nuclear stain at 1:40000 for 2 hours at room temperature. Localization: Mitochondrion matrix. Magnification: 100x.(A) DAPI (blue) nuclear stain. (B) Anti-Hsp60 Antibody. (C) Composite. Heat Shocked at 42°C for 1h.

Publishing research using Anti-HSP60 Antibody (A305092)? Please let us know so that we can list the citation on this page.