Anti-HSP90 Antibody (A305030)

Name: Anti-HSP90 Antibody
Brand: Antibodies.com
SKU: A305030
Price: 425.0 USD
Availability: InStock

Enlarge Image

$425

Rabbit polyclonal antibody to HSP90 for WB, IHC, ICC/IF, IP and ELISA.

100% Guarantee

Price Match Guarantee

Delivery

Freight Charges

$40 to United States

Lead Time

5-8 Business Days

Contact Us

Telephone

+1 (314) 370-6046

orders@antibodies.com

Guarantees

100% Quality Guarantee

Price Match Guarantee

Specifications

Name

Anti-HSP90 Antibody

Description

Rabbit polyclonal antibody to HSP90.

Applications

WB, IHC, ICC/IF, IP, ELISA

Dilutions

WB: 1:500, IHC: 1:100, ICC/IF: 1:100

Reactivity

Human, Mouse, Rat

Immunogen

Full length Human Hsp90 protein.

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Conjugate

Unconjugated

Purification

Protein A purification.

Concentration

1 mg/ml

Molecular Weight

~90 kDa

Product Form

Liquid

Formulation

Supplied in Phosphate Buffered Saline, pH 7.4, with 50% Glycerol and 0.09% Sodium Azide.

Storage

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

Synonyms

Heat shock 86 kDa, Heat shock protein 90kDa alpha cytosolic class A member 1, Heat shock protein 90kDa alpha cytosolic class B member 1, Heat shock protein HSP 90 alpha, Heat shock protein HSP 90 beta, Heat shock protein HSP 90-alpha, HS90A_HUMAN, HSP 84, HSP 86, Hsp 90, HSP86, HSP90A, HSP90AA1, HSP90AB1, HSP90B, HSPC1, HSPC2, HSPCAL1, HSPCAL4, Renal carcinoma antigen NY-REN-38

Isotype Controls

Suitable Secondaries

See all Anti-Rabbit IgG Secondaries →

Disclaimer

This product is for research use only. It is not intended for diagnostic or therapeutic use.

Scientific Validation Data (4)

Enlarge Image

Figure 1: Immunofluorescence - Anti-HSP90 Antibody (A305030)

Immunocytochemistry/Immunofluorescence analysis of human heat shocked cervical cancer cell line (HeLa), fixed in 2% formaldehyde for 20 minutes at room temperature, using Anti-HSP90 Antibody (A305030), at 1:100 for 12 hours at 4°C. The secondary antibody used was APC Goat Anti-Rabbit (red) at 1:200 for 2 hours at room temperature. Counterstain: DAPI (blue) nuclear stain at 1:40000 for 2 hours at room temperature. Localization: Cytoplasm. Magnification: 20x.(A) DAPI (blue) nuclear stain. (B) Anti-Hsp90 Antibody. (C) Composite. Heat Shocked at 42°C for 1h.

Enlarge Image

Figure 2: Immunohistochemistry - Anti-HSP90 Antibody (A305030)

Immunohistochemistry analysis of mouse backskin, fixed in Bouin's fixative solution. The Primary Antibody used was Anti-HSP90 Antibody (A305030) at 1:100 for 1 hour at room temperature. The secondary antibody used was FITC Goat Anti-Rabbit (green) at 1:50 for 1 hour at room temperature. Localization: Cytoplasm.

Enlarge Image

Figure 3: Western Blot - Anti-HSP90 Antibody (A305030)

Western blot analysis of human Cell line lysates showing detection of HSP90 protein using Anti-HSP90 Antibody (A305030) at 1:10,000 for 2 hours at room temperature. Load: 2 µgHsp90beta. Block: 1.5% BSA for 30 minutes at room temperature. The secondary antibody used was Donkey Anti-Rabbit IgG: HRP for 1 hour at room temperature.

Enlarge Image

Figure 4: Immunofluorescence - Anti-HSP90 Antibody (A305030)

Immunocytochemistry/Immunofluorescence analysis of human heat shocked cervical cancer cell line (HeLa), fixed in 2% formaldehyde for 20 minutes at room temperature, using Anti-HSP90 Antibody (A305030), at 1:100 for 12 hours at 4°C. The secondary antibody used was FITC Goat Anti-Rabbit (green) at 1:200 for 2 hours at room temperature. Counterstain: DAPI (blue) nuclear stain at 1:40000 for 2 hours at room temperature. Localization: Cytoplasm. Magnification: 100x.(A) DAPI (blue) nuclear stain. (B) Anti-Hsp90 Antibody. (C) Composite. Heat Shocked at 42°C for 1h.

Publishing research using Anti-HSP90 Antibody (A305030)? Please let us know so that we can list the citation on this page.