Supplied in Phosphate Buffered Saline, pH 7.3, with 50% Glycerol and 0.01% Thiomersal.
Storage
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze/thaw cycles.
Synonyms
Heat shock 86 kDa, Heat shock protein family C member 1, Heat shock protein HSP 90-alpha, HSP 86, HSP86, HSP90A, HSP90AA1, HSPC1, HSPCA, LAP-2, Lipopolysaccharide-associated protein 2, LPS-associated protein 2, Renal carcinoma antigen NY-REN-38
Western blot analysis of extracts of various cell lines, using Anti-Hsp90 alpha Antibody (A87729) at 1:1,000 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 30s.
Immunohistochemistry analysis of paraffin-embedded rat testis using Anti-Hsp90 alpha Antibody (A87729) at a dilution of 1:200 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.
Immunohistochemistry analysis of paraffin-embedded rat brain using Anti-Hsp90 alpha Antibody (A87729) at a dilution of 1:200 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.
Immunohistochemistry analysis of paraffin-embedded mouse testis using Anti-Hsp90 alpha Antibody (A87729) at a dilution of 1:200 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.
Immunohistochemistry analysis of paraffin-embedded mouse brain using Anti-Hsp90 alpha Antibody (A87729) at a dilution of 1:200 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.
Immunofluorescence analysis of C6 cells using Anti-Hsp90 alpha Antibody (A87729) at a dilution of 1:100. DAPI was used to stain the cell nuclei (blue).
![Western Blot - Anti-HSP90 alpha Antibody [2G5.G3] (A305031) - Antibodies.com](https://cdn.antibodies.com/image/catalog/305/A305031_1.png?profile=product_alternative)
![Immunohistochemistry - Anti-HSP90 alpha Antibody [Hyb-K41009] (A305061) - Antibodies.com](https://cdn.antibodies.com/image/catalog/305/A305061_1.png?profile=product_alternative)
