Human Amylase Alpha ELISA Kit (A73853)

Human Amylase Alpha ELISA Kit

Sandwich (quantitative)

This Human Amylase Alpha ELISA Kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of Human Amylase Alpha in plasma, tissue homogenates and other biological fluids.

An antibody specific for Amylase Alpha has been coated on a microplate. Standards and samples are pipetted into the wells and the Amylase Alpha present in each sample is bound to the wells by the immobilised antibody. The wells are washed and Anti-Amylase Alpha Antibody (Biotin) is added. After washing away the unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted into the wells. The wells are washed again, before a TMB substrate solution is added to the wells and colour develops in proportion to the amount of Amylase Alpha bound. The Stop Solution changes the colour from blue to yellow, and the intensity of the colour is measured at 450 nm.

Microplate

Colorimetric

< 9.4 mIU/ml

15.6 - 1000 mIU/ml

Human

4°C for 6 months.

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This product is for research use only. It is not intended for diagnostic or therapeutic use.