Human Apolipoprotein A1 ELISA Kit is based on the quantitative sandwich enzyme-linked immunosorbent assay technique to measure concentration of human Apo A1 in the samples. A monoclonal antibody specific for human Apo A1 has been immobilized onto microwells. Standard or samples are pipetted into the wells, followed by the addition of biotin-linked monoclonal antibody specific for Apo A1, and Apo A1 present is bound by the immobilized antibody and detect antibody following the first incubation. After removal of any unbound substances, streptavidin-HRP is added for a second incubation. After washing, substrate solution reacts with HRP and color develops in proportion to the amount of Apo A1 bound by the immobilized antibody. The color development is stopped by addition of acid and the optical density value is measured by microplate reader.
Platform
Microplate
Detection Type
Colorimetric
Sample Type
Cell culture supernatant, serum and plasma.
Sensitivity
0.73 ng/ml
Product Range
3.13 - 200 ng/ml
Specificity
This kit can assay both natural and recombinant human Apo A1.
Reactivity
Human
Cross Reactivity
This assay has shown no significant cross-reactivity.
Storage
Store at 2 - 8°C.
Synonyms
Apo-AI, ApoA I, ApoA-I, APOA1, APOA1_HUMAN, Apolipoprotein A I, Apolipoprotein A-I, Apolipoprotein A-I(1-242), Apolipoprotein AI, Brp14, Ltw1, Lvtw1, Sep1, Sep2, Truncated apolipoprotein A-I
Disclaimer
This product is for research use only. It is not intended for diagnostic or therapeutic use.
