Human ATP13A2 ELISA Kit (A74668)

This product is discontinued

Human ATP13A2 ELISA Kit (A74668) has been discontinued and is no longer available.

View all available products.

Name
Human ATP13A2 ELISA Kit
Assay Type
Sandwich (quantitative)
Principle of Assay
This Human ATP13A2 ELISA Kit is an in vitro competitive enzyme-linked immunosorbent assay for the quantitative measurement of ATP13A2 in plasma, tissue homogenates and other biological fluids.

A microplate has been precoated with Anti-ATP13A2 Antibodies. Samples and the ATP13A2-HRP conjugate are added to the wells, where ATP13A2 in the sample competes with the added ATP13A2-HRP for antibody binding. After incubation, the wells are washed to remove unbound material and TMB substrate is added. The TMB substrate is catalyzed by HRP to produce blue colouration. The reaction is terminated by addition of Stop Solution which stops the colour development and produces a colour change from blue to yellow. The intensity of the signal is inversely proportional to the amount of ATP13A2 in the sample and the intensity is measured at 45 nm.
Platform
Pre-coated Microplate (12 x 8 well strips)
Detection Type
Colorimetric
Instrument
Colorimetric Microplate Reader
Sample Type
Serum, plasma, tissue homogenates, and other biological fluids.
Sensitivity
0.188 ng/ml
Product Range
0.313-20 ng/ml
Assay Time
4h 30m
Reactivity
Human
Recovery
Sample Type n Range Average
Serum 5 89% - 101% 95%
EDTA Plasma 5 89% - 98% 92%
Heparin Plasma 5 89% - 102% 95%
Linearity
Sample Type n 1:2 1:4 1:8
Serum 5 85-99% 86-103% 85-101%
EDTA Plasma 5 82-99% 82-94% 83-93%
Heparin Plasma 5 81-97% 83-97% 87-98%
Precision
Intra- assay CV: < 8%, Inter-assay CV: < 10%
General Notes
Information online is representative. Refer to the Instructions for Use inside the kit.
Storage
Store at +4°C. Do not use past expiration date!
Disclaimer
This product is for research use only. It is not intended for diagnostic or therapeutic use.
Publishing research using Human ATP13A2 ELISA Kit (A74668)? Please let us know so that we can list the citation on this page.
Top