Human BRCA2 ELISA Kit (A76219)

This product is discontinued

Human BRCA2 ELISA Kit (A76219) has been discontinued and is no longer available.

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Name
Human BRCA2 ELISA Kit
Assay Type
Sandwich (quantitative)
Principle of Assay
This Human BRCA2 ELISA Kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of Human BRCA2 in plasma, tissue homogenates and other biological fluids.

An antibody specific for BRCA2 has been coated on a microplate. Standards and samples are pipetted into the wells and the BRCA2 present in each sample is bound to the wells by the immobilised antibody. The wells are washed and Anti-BRCA2 Antibody (Biotin) is added. After washing away the unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted into the wells. The wells are washed again, before a TMB substrate solution is added to the wells and colour develops in proportion to the amount of BRCA2 bound. The Stop Solution changes the colour from blue to yellow, and the intensity of the colour is measured at 450 nm.
Platform
Microplate
Detection Type
Colorimetric
Sample Type
Plasma, tissue homogenates and other biological fluids.
Sensitivity
< 0.188 ng/ml
Product Range
0.313 - 20 ng/ml
Reactivity
Human
Storage
4°C for 6 months.
Synonyms
BRCA 2, BRCA1/BRCA2 containing complex subunit 2, BRCA2, DNA repair associated, BRCA2_HUMAN, BRCC 2, BRCC2, Breast and ovarian cancer susceptibility gene early onset, breast and ovarian cancer susceptibility protein 2, Breast cancer 2 early onset, Breast Cancer 2 tumor suppressor, Breast cancer susceptibility protein BRCA2, Breast cancer type 2 susceptibility protein, BROVCA2, FACD, FAD, FAD 1, FAD1, FANCB, FANCD, FANCD 1, FANCD1, FANCD1 gene, Fanconi anemia complementation group D1, Fanconi anemia group D1 protein, GLM3, mutant BRCA2, OTTHUMP00000018803, OTTHUMP00000042401, PNCA2, XRCC11
Disclaimer
This product is for research use only. It is not intended for diagnostic or therapeutic use.
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