Human Cathepsin D ELISA Kit (A530)

Human Cathepsin D ELISA Kit

Sandwich (quantitative)

This Cathepsin D enzyme linked immunosorbent assay (ELISA) applies a technique called a quantitative sandwich immunoassay. The microtiter plate has been pre-coated with an Anti-Cathepsin D Antibody. Standards or samples are then added to the microtire plate wells and Cathepsin D, if present, will bind to the antibody pre-coated on the wells. In order to quantify the amount of Cathepsin D present in the sample, Anti-Cathepsin D Antibody (Biotin) is added to each well to "sandwich" the Cathepsin D immobilised on the plate. The microtiter plate then undergoes incubation, followed by thorough washing of the wells to remove all unbound components. Next, Avidin-Biotin-Peroxidase Complex is added to each well for a short incubation period, followed by thorough washing of the wells to remove all unbound conjugates. Next, a TMB (3,3',5,5' tetramethyl-benzidine) substrate solution is added to each well. The enzyme (HRP) and substrate are allowed to react over a short incubation period. Only those wells that contain Cathepsin D will exhibit a change in colour. The enzyme-substrate reaction is terminated by the addition of a stop solution and the colour change is measured spectrophotometrically at a wavelength of 450nm ± 2nm.

Microplate

Serum, plasma, and cell culture supernatants.

< 10 pg/ml

156-10,000 pg/ml

Human

Store at 2-8°C for 4 months or at -20°C for 8 months.

CatD, CATD_HUMAN, Cathepsin D heavy chain, CD, Ceroid lipofuscinosis neuronal 10, CLN10, CPSD, CTSD, Epididymis secretory sperm binding protein Li 130P, HEL S 130P, Lysosomal aspartyl peptidase, Lysosomal aspartyl protease, MGC2311