Human Centromere Protein F ELISA Kit (A4984)

Human Centromere Protein F ELISA Kit

Sandwich (quantitative)

The microtiter plate provided in this kit has been pre-coated with an antibody specific to the index. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific to the index. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain the index, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of the index in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Microplate

Colorimetric

0.046 ng/ml

0.156 - 10 ng/ml

1 - 4.5 Hours

Human

AH antigen, Cell cycle dependent 350K nuclear protein, CENF, CENP F, CENP F kinetochore protein, CENP-F, CENPF, CENPF kinetochore protein, CENPF_HUMAN, Centromere protein F 350/400ka, Centromere protein F, 350/400kDa, CILD31, Hcp 1, Hcp1, Kinetochore protein CENP F, Kinetochore protein CENPF, Mitosin, PRO1779, STROMS