Figure 1: Western Blot - Anti-Human Coronavirus Spike glycoprotein Antibody (A308071)
Western blot analysis of extracts of Human coronavirus (HCoV-OC43) Spike Protein (S1+S2 ECDHis Tag), using Anti-Human Coronavirus Spike glycoprotein Antibody (A308071) at 1:1,000 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Enhanced Kit (RM00021). Exposure time: 180s.
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