Human Cytochrome P450 2C9 ELISA Kit (A82306)

Human Cytochrome P450 2C9 ELISA Kit

Sandwich (quantitative)

The microtiter plate provided in this kit has been pre-coated with an antibody specific to the index. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific to the index. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain the index, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of the index in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Microplate

Colorimetric

0.234 ng/ml

0.625 - 40 ng/ml

1 - 4.5 Hours

Human

(R)-limonene 6-monooxygenase, (S)-limonene 6-monooxygenase, (S)-limonene 7-monooxygenase, CP2C9_HUMAN, CPC9, CYP2C, CYP2C10, CYP2C9, CYPIIC9, cytochrome P-450 S-mephenytoin 4-hydroxylase, Cytochrome P-450MP, Cytochrome P450 MP-4, Cytochrome P450 MP-8, Cytochrome P450 PB-1, Cytochrome P450, family 2, subfamily C, polypeptide 9, Cytochrome p4502C9, flavoprotein-linked monooxygenase, MGC149605, MGC88320, microsomal monooxygenase, OTTHUMP00000020135, P450 MP, P450 PB 1, P450 PB1, P450IIC9, P450MP, S mephenytoin 4 hydroxylase, S-mephenytoin 4-hydroxylase, xenobiotic monooxygenase