Mouse monoclonal [BE5] antibody to Human IgE (FITC).
Specificity
This antibody recognizes human IgE. It recognizes a different epitope to Clone ID's 4G7 and 4H10.
Applications
Flow Cytometry
Dilutions
Flow Cytometry: 5 µg/ml.
Reactivity
Human
Immunogen
Purified human IgE.
Host
Mouse
Clonality
Monoclonal
Clone ID
BE5
Isotype
IgG1
Conjugate
FITC
Excitation: 490nm, Emission: 525nm
Purification
This antibody is conjugated with Fluorescein Isothiocyanate (FITC) under optimum conditions. The conjugate is purified by size-exclusion chromatography; removing unconjugated antibody and free fluorochrome.
Concentration
100 µg/ml
Product Form
Liquid
Formulation
Supplied in Phosphate Buffered Saline, pH 7.4, with 15 mM Sodium Azide.
Storage
Shipped at 4°C. Store in the dark at 2-8°C. Do not freeze!
Flow Cytometry analysis of basophil activation upon stimulation of normal (heparin-treated) whole blood with combination of IL-3 and Goat Anti-IgE Antibody. Combination of Anti-IgE Antibody [BE5] (FITC) and Anti-CD63 Antibody [MEM-259] (PE-Dyomics 647) was used (analysis in basophil window). Staining of non-stimulated (control) sample.
Flow Cytometry analysis of basophil activation upon stimulation of normal (heparin-treated) whole blood with combination of IL-3 and Goat Anti-IgE Antibody. Combination of Anti-IgE Antibody [BE5] (FITC) and Anti-CD63 Antibody [MEM-259] (PE-Dyomics 647) was used (analysis in basophil window). Staining of IgE stimulated sample.
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