Recombinant Anti-Human IgE Antibody [RM122] (Biotin) (A121279)

Name: Anti-Human IgE Antibody [RM122] (Biotin)
Brand: Antibodies.com
SKU: A121279
Price: 480.0 USD
Availability: InStock

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$480

Recombinant rabbit monoclonal [RM122] antibody to Human IgE (Biotin) for Flow Cytometry and ELISA.

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Delivery

Freight Charges

$40 to United States

Lead Time

4-6 Business Days

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+1 (314) 370-6046

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Recombinant Antibody

Specifications

Name

Anti-Human IgE Antibody [RM122] (Biotin)

Description

Recombinant rabbit monoclonal [RM122] antibody to Human IgE (Biotin).

Specificity

This antibody recognizes human IgE.

Applications

Flow Cytometry, ELISA

Dilutions

ELISA: 10-100 ng/well (Capture) and 0.01-0.1 µg/ml (Detection), ICC: 0.5-2 µg/ml, IHC: 0.5-2 µg/ml

Reactivity

Human

Cross Reactivity

There is no cross reactivity with human IgG, IgM, IgD, or IgA.

Immunogen

Human IgE.

Host

Rabbit

Clonality

Monoclonal

Clone ID

RM122

Isotype

IgG

Conjugate

Biotin

Purification

Protein A chromatography.

Concentration

1 mg/ml

Product Form

Liquid

Formulation

Supplied in Phosphate Buffered Saline with 50% Glycerol, 1% BSA, and 0.09% Sodium Azide.

Storage

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

Synonyms

Ig epsilon chain C region, Igh2, IGHE, IGHEP1, Immunoglobulin heavy constant epsilon

Disclaimer

This product is for research use only. It is not intended for diagnostic or therapeutic use.

Scientific Validation Data (3)

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Figure 1: Anti-Human IgE Antibody [RM122] (Biotin) (A121279)

Sandwich ELISA using Anti-Human IgE Antibody [RM122] as the capture antibody (25ng/well), and Anti-Human Ig Light Chain Antibody [RM129] (Biotin) as the detection antibody, followed by an AP conjugated streptavidin.

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Figure 2: Anti-Human IgE Antibody [RM122] (Biotin) (A121279)

ELISA of human immunoglobulins shows Anti-Human IgE Antibody [RM122] reacts only to human IgE? from human myeloma plasma and the IgE from human plasma. No cross reactivity with Human IgG, IgM, IgD, or IgA. The plate was coated with 50ng/well of different immunoglobulins. 200ng/ml, 50ng/ml or 10ng/ml of Anti-Human IgE Antibody [RM122] was used as the primary antibody. An alkaline phosphatase conjugated anti-rabbit IgG as the secondary antibody.

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Figure 3: Anti-Human IgE Antibody [RM122] (Biotin) (A121279)

A titer ELISA using Anti-Human IgE Antibody [RM122]. The plate was coated with different amounts of human IgE. A serial dilution of Anti-Human IgE Antibody [RM122] was used as the primary antibody. An alkaline phosphatase conjugated anti-rabbit IgG as the secondary antibody.

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