Human IGFBP1 ELISA Kit (A595)

Human IGFBP1 ELISA Kit

Sandwich (quantitative)

This IGFBP1 enzyme linked immunosorbent assay (ELISA) applies a technique called a quantitative sandwich immunoassay. The microtiter plate has been pre-coated with an Anti-IGFBP1 Antibody. Standards or samples are then added to the microtire plate wells and IGFBP1, if present, will bind to the antibody pre-coated on the wells. In order to quantify the amount of IGFBP1 present in the sample, Anti-IGFBP1 Antibody (Biotin) is added to each well to "sandwich" the IGFBP1 immobilised on the plate. The microtiter plate then undergoes incubation, followed by thorough washing of the wells to remove all unbound components. Next, Avidin-Biotin-Peroxidase Complex is added to each well for a short incubation period, followed by thorough washing of the wells to remove all unbound conjugates. Next, a TMB (3,3',5,5' tetramethyl-benzidine) substrate solution is added to each well. The enzyme (HRP) and substrate are allowed to react over a short incubation period. Only those wells that contain IGFBP1 will exhibit a change in colour. The enzyme-substrate reaction is terminated by the addition of a stop solution and the colour change is measured spectrophotometrically at a wavelength of 450nm ± 2nm.

Microplate

Serum, plasma, body fluids, tissue lysates, and cell culture supernatants.

< 1 pg/ml

31.2-2000 pg/ml

Human

Store at 2-8°C for 6 months or at -20°C for 12 months.

AFBP, Alpha pregnancy associated endometrial globulin, Amniotic fluid binding protein, Binding protein 25, Binding protein 26, Binding protein 28, Growth hormone independent binding protein, hIGFBP 1, hIGFBP1, IBP 1, IBP-1, IBP1, IBP1_HUMAN, IGF binding protein 1, IGF BP25, IGF-binding protein 1, IGFBP 1, IGFBP-1, Insulin like growth factor binding protein 1, Insulin-like growth factor-binding protein 1, Placental protein 12, PP 12, PP12