Recombinant Anti-Human IgG2 Antibody [RM118] (A121338)

Name: Anti-Human IgG2 Antibody [RM118]
Brand: Antibodies.com
SKU: A121338
Price: 480.0 USD
Availability: InStock

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$480

Recombinant rabbit monoclonal [RM118] antibody to Human IgG2 for Flow Cytometry and ELISA.

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Delivery

Freight Charges

$40 to United States

Lead Time

4-6 Business Days

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Recombinant Antibody

Specifications

Name

Anti-Human IgG2 Antibody [RM118]

Description

Recombinant rabbit monoclonal [RM118] antibody to Human IgG2.

Specificity

This antibody recognizes the heavy chain of human IgG2.

Applications

Flow Cytometry, ELISA

Dilutions

ELISA: 50-200 ng/well (Capture) and 0.05-0.2 µg/ml (Detection), ICC: 0.5-2 µg/ml, IHC: 0.5-2 µg/ml

Reactivity

Human

Cross Reactivity

There is no cross reactivity with human IgG1, IgG3, IgG4, IgM, IgA, IgD, IgE, monkey IgG, mouse IgG, rat IgG, or goat IgG.

Immunogen

Human IgG2.

Host

Rabbit

Clonality

Monoclonal

Clone ID

RM118

Isotype

IgG

Conjugate

Unconjugated

Purification

Protein A chromatography.

Concentration

1 mg/ml

Product Form

Liquid

Formulation

Supplied in Phosphate Buffered Saline with 50% Glycerol, 1% BSA, and 0.09% Sodium Azide.

Storage

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

Synonyms

Ig gamma-2 chain C region, IGHG2, immunoglobulin Gm2, immunoglobulin heavy constant gamma 2 (G2m marker), immunoglobulin heavy constant, gamma 2

Isotype Controls

Suitable Secondaries

See all Anti-Rabbit IgG Secondaries →

Disclaimer

This product is for research use only. It is not intended for diagnostic or therapeutic use.

Scientific Validation Data (5)

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Figure 1: Anti-Human IgG2 Antibody [RM118] (A121338)

Detection of human IgG2 in monkey serum, using Anti-Human IgG2 Antibody [RM118] (capture antibody) and Anti-Human Ig Light Chain Antibody [RM129] (Biotin) (detection antibody) as a Sandwich ELISA pair. HRP conjugated streptavidin and TMB were used to yield the colorimetric reaction.

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Figure 2: Anti-Human IgG2 Antibody [RM118] (A121338)

Sandwich ELISA using Anti-Human IgG2 Antibody [RM118] as the capture antibody, and Anti-Human Ig Light Chain Antibody [RM129] (Biotin) as the detection antibody, followed by an AP conjugated streptavidin.

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Figure 3: Anti-Human IgG2 Antibody [RM118] (A121338)

ELISA showing Anti-Human IgG2 Antibody [RM118] reacts only to human IgG2, and not to any other IgG subclasses (IgG1, IgG3, or IgG4), and there is no cross reactivity to IgM, IgA, IgD, IgE, mouse IgG, rat IgG, or goat IgG.

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Figure 4: Anti-Human IgG2 Antibody [RM118] (A121338)

A titer ELISA using Anti-Human IgG2 Antibody [RM118]. The plate was coated with different amounts of human IgG2. A serial dilution of Anti-Human IgG2 Antibody [RM118] was used as the primary antibody. An alkaline phosphatase conjugated anti-rabbit IgG as the secondary antibody.