Sandwich ELISA using Anti-Human IgG4 Antibody [RM120] as the capture antibody, and Anti-Human Ig Light Chain Antibody [RM129] (Biotin) as the detection antibody, followed by an AP conjugated streptavidin.
Sandwich ELISA using Anti-Human IgG4 Antibody [RM120] as the capture antibody, and Anti-Human Ig Light Chain Antibody [RM129] (Biotin) as the detection antibody, followed by an AP conjugated streptavidin.
Figure 3: Western Blot - Anti-Human IgG4 Antibody [RM120] (Biotin) (A121272)
Western blot of human, mouse, rat, and goat IgG shows Anti-Human IgG4 Antibody [RM120] (Biotin) reacts to human IgG4, in both whole molecule (~150kDa, non-reduced) and heavy chain (~50kDa, reduced) forms. There is no cross reactivity with other isotypes of human IgG, mouse IgG, rat IgG, or goat IgG.
A titer ELISA using Anti-Human IgG4 Antibody [RM120]. The plate was coated with different amounts of human IgG4. A serial dilution of Anti-Human IgG4 Antibody [RM120] was used as the primary antibody. An alkaline phosphatase conjugated anti-rabbit IgG as the secondary antibody.
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