Human Interleukin-2 ELISA Kit (A33010)

Human Interleukin-2 ELISA Kit

Sandwich (quantitative)

This assay employs the quantitative sandwich enzyme immunoassay technique. A monoclonal antibody specific for IL-2 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any IL-2 present is bound by the immobilized antibody. After washing away any unbound substances, an enzyme-linked polyclonal antibody specific for IL-2 is added to the wells. Following a wash to remove any unbound antibody-enzyme reagent, a substrate solution is added to the wells and colour develops in proportion to the amount of IL-2 bound in the initial step. The colour development is stopped and the intensity of the colour is measured.

In order to measure the concentration of IL-2 in the samples this kit includes two calibration diluents (Calibrator Diluent I for serum/plasma testing and Calibrator Diluent II for cell culture supernatant testing.) According to the testing system, the provided standard is diluted (2-fold) with the appropriate Calibrator Diluent and assayed at the same time as the samples. This allows the operator to produce a standard curve of Optical Density (O.D) versus IL-2 concentration (pg/mL). The concentration of IL-2 in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Microplate

Colorimetric

< 6.7 pg/ml

6.7-2500 pg/ml

4h 30m

This sandwich ELISA can detect both natural and recombinant human IL-2.

Human

This assay has shown no cross-reactivity with other cytokines tested.

Store at 2-8°C.

Aldesleukin, IL 2, IL-2, IL2, IL2_HUMAN, Interleukin 2, Interleukin2, Involved in regulation of T cell clonal expansion, Lymphokine , OTTHUMP00000164090, POIL2, T cell growth factor, T-cell growth factor, TCGF