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Anti-Human Kappa Light Chain Antibody [ARC2527] (A307148)

Western Blot - Anti-Human Kappa Light Chain Antibody [ARC2527] (A307148) - Antibodies.com Immunohistochemistry - Anti-Human Kappa Light Chain Antibody [ARC2527] (A307148) - Antibodies.com
$470
Product Datasheet

Rabbit monoclonal [ARC2527] antibody to Human Kappa Light Chain for WB and IHC.

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$40 to United States
Lead Time
5-8 Business Days

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Name
Anti-Human Kappa Light Chain Antibody [ARC2527]
Description
Rabbit monoclonal [ARC2527] antibody to Human Kappa Light Chain.
Applications
WB, IHC
Dilutions
WB: 1:500-1:1,000, IHC: 1:50-1:200
Reactivity
Human
Immunogen
Recombinant fusion protein containing a sequence corresponding to amino acids 1-117 of human Kappa light chain (P01601).
Sequence
MDMRVLAQLLGLLLLCFPGARCDIQMTQSPSSLSASVGDRVTITCRASQGISSWLAWYQQKPEKAPKSLIYAASSLQSGVPSRFSGSGSGTDFTLTISSLQPEDFATYYCQQYNSYP
Host
Rabbit
Clonality
Monoclonal
Clone ID
ARC2527
Isotype
IgG
Conjugate

Unconjugated

Purification
Affinity purification.
Molecular Weight
25 kDa
Product Form
Liquid
Formulation
Supplied in Phosphate Buffered Saline, pH 7.3, with 50% Glycerol, 0.05% BSA, and 0.02% Sodium Azide.
Storage
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Isotype Controls
Suitable Secondaries
Disclaimer
This product is for research use only. It is not intended for diagnostic or therapeutic use.
Western Blot - Anti-Human Kappa Light Chain Antibody [ARC2527] (A307148) - Antibodies.comEnlarge Image
Figure 1: Western Blot - Anti-Human Kappa Light Chain Antibody [ARC2527] (A307148)
Western blot analysis of extracts of Human plasma cells, using Anti-Human Kappa Light Chain Antibody [ARC2527] (A307148) at 1:1,000 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 1s.
Immunohistochemistry - Anti-Human Kappa Light Chain Antibody [ARC2527] (A307148) - Antibodies.comEnlarge Image
Figure 2: Immunohistochemistry - Anti-Human Kappa Light Chain Antibody [ARC2527] (A307148)
Immunohistochemistry analysis of paraffin-embedded human tonsil using Anti-Human Kappa Light Chain Antibody [ARC2527] (A307148) at a dilution of 1:100 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.
Publishing research using Anti-Human Kappa Light Chain Antibody [ARC2527] (A307148)? Please let us know so that we can list the citation on this page.

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