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Anti-Human Kappa Light Chain Antibody [TB28-2] (PE-Cyanine 7) (A285796)

Flow Cytometry - Anti-Human Kappa Light Chain Antibody [TB28-2] (PE-Cyanine 7) (A285796) - Antibodies.com Flow Cytometry - Anti-Human Kappa Light Chain Antibody [TB28-2] (PE-Cyanine 7) (A254436) - Antibodies.com Flow Cytometry - Anti-Human Kappa Light Chain Antibody [TB28-2] (PE-Cyanine 7) (A285796) - Antibodies.com
$590
Product Datasheet

Mouse monoclonal [TB28-2] antibody to Human Kappa Light Chain (PE-Cyanine 7) for Flow Cytometry.

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Alternative Formats and ConjugatesAlternative Formats

Freight Charges
$40 to United States
Lead Time
4-6 Business Days

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Name
Anti-Human Kappa Light Chain Antibody [TB28-2] (PE-Cyanine 7)
Description
Mouse monoclonal [TB28-2] antibody to Human Kappa Light Chain (PE-Cyanine 7).
Specificity
This antibody recognizes human Ig kappa light chains of both secreted and cell surface immunoglobulin. It also detects free kappa light chains.
Applications
Flow Cytometry
Dilutions
Flow Cytometry: The reagent is designed for analysis of human blood cells using 4 µl reagent / 100 µl of whole blood or 106 cells in a suspension. The content of a vial (0.4 ml) is sufficient for 100 tests; Extracellular and Intracellular staining.
Reactivity
Human
Immunogen
Human IgG-kappa myeloma protein.
Host
Mouse
Clonality
Monoclonal
Clone ID
TB28-2
Isotype
IgG1
Light Chains
kappa
Conjugate

PE-Cyanine 7

Excitation: 565nm, Emission: 778nm

Purification
This antibody is conjugated with tandem dye PE-Cyanine 7 under optimum conditions. The conjugate is purified by size-exclusion chromatography; removing unconjugated antibody and free fluorochrome.
Product Form
Liquid
Formulation
Supplied in Phosphate Buffered Saline, pH 7.4, with 15 mM Sodium Azide.
Storage
Shipped at 4°C. Store in the dark at 2-8°C. Do not freeze!
Isotype Controls
Disclaimer
This product is for research use only. It is not intended for diagnostic or therapeutic use.
Flow Cytometry - Anti-Human Kappa Light Chain Antibody [TB28-2] (PE-Cyanine 7) (A285796) - Antibodies.comEnlarge Image
Figure 1: Flow Cytometry - Anti-Human Kappa Light Chain Antibody [TB28-2] (PE-Cyanine 7) (A285796)
Flow cytometric analysis of human peripheral whole blood stained with Anti-Human Kappa Light Chain Antibody [TB28-2] (PE-Cyanine 7) (4 µl reagent / 100 µl of peripheral whole blood).
Flow Cytometry - Anti-Human Kappa Light Chain Antibody [TB28-2] (PE-Cyanine 7) (A254436) - Antibodies.comEnlarge Image
Figure 2: Flow Cytometry - Anti-Human Kappa Light Chain Antibody [TB28-2] (PE-Cyanine 7) (A285796)
Flow cytometry multicolor surface staining of human lymphocytes stained with Anti-Human Kappa Light Chain Antibody [TB28-2] (PE-Cyanine 7) (4 µl reagent / 100 µl of peripheral whole blood) and Anti-CD19 Antibody [LT19] (APC) (4 µl reagent / 100 µl of peripheral whole blood).
Flow Cytometry - Anti-Human Kappa Light Chain Antibody [TB28-2] (PE-Cyanine 7) (A285796) - Antibodies.comEnlarge Image
Figure 3: Flow Cytometry - Anti-Human Kappa Light Chain Antibody [TB28-2] (PE-Cyanine 7) (A285796)
Separation of human Ig Kappa Light Chain positive B cells (red-filled) from Ig Kappa Light Chain negative CD19 negative (black-dashed) in flow cytometry analysis (surface staining) of human peripheral whole blood stained with Anti-Human Kappa Light Chain Antibody [TB28-2] (PE-Cyanine 7) (4 µl reagent / 100 µl of peripheral whole blood).
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