Human L Selectin ELISA Kit (A646)

Human L Selectin ELISA Kit

Sandwich (quantitative)

This L Selectin enzyme linked immunosorbent assay (ELISA) applies a technique called a quantitative sandwich immunoassay. The microtiter plate has been pre-coated with an Anti-L Selectin Antibody. Standards or samples are then added to the microtire plate wells and L Selectin, if present, will bind to the antibody pre-coated on the wells. In order to quantify the amount of L Selectin present in the sample, Anti-L Selectin Antibody (Biotin) is added to each well to "sandwich" the L Selectin immobilised on the plate. The microtiter plate then undergoes incubation, followed by thorough washing of the wells to remove all unbound components. Next, Avidin-Biotin-Peroxidase Complex is added to each well for a short incubation period, followed by thorough washing of the wells to remove all unbound conjugates. Next, a TMB (3,3',5,5' tetramethyl-benzidine) substrate solution is added to each well. The enzyme (HRP) and substrate are allowed to react over a short incubation period. Only those wells that contain L Selectin will exhibit a change in colour. The enzyme-substrate reaction is terminated by the addition of a stop solution and the colour change is measured spectrophotometrically at a wavelength of 450nm ± 2nm.

Microplate

Serum, plasma, body fluids, tissue lysates, and cell culture supernatants.

< 5 pg/ml

62.5-4000 pg/ml

Human

Store at 2-8°C for 6 months or at -20°C for 12 months.

A.11 , AI528707, CD62 antigen ligand , CD62 antigen-like family member L, CD62L, gp90 MEL , gp90-MEL, hLHRc, IgA nephropathy, susceptibility to, included , L-selectin, LAM-1, LAM1 , LECAM1 , Leu8, Leukocyte adhesion molecule 1 , Leukocyte surface antigen Leu-8, Leukocyte surface antigen Leu8 , Leukocyte-endothelial cell adhesion molecule 1 , LNHR, LSEL, Ly-22 , Ly-m22 , Lyam-1, LYAM1 , LYAM1_HUMAN, Lymph node homing receptor , Lymphocyte adhesion molecule 1, Lymphocyte antigen 22 , Lymphocyte surface MEL-14 antigen , MEL-14, Pln homing receptor, PLNHR , Selectin L , Selectin, lymphocyte , SELL, TQ1