Recombinant Anti-Human Nuclear Antigen Antibody [235-1R] (A278107)

Anti-Human Nuclear Antigen Antibody [235-1R]

Recombinant rabbit monoclonal [235-1R] antibody to Human Nuclear Antigen.

This antibody is an excellent marker for human cells in xenographic model research. It reacts specifically with human cells. It is a part of a new panel of reagents, which recognizes subcellular organelles or compartments of human cells. These markers may be useful in identification of these organelles in cells, tissues, and biochemical preparations. MAb 235-1R recognizes an antigen associated with the nuclei in human cells. It can be used to stain the nuclei in cell or tissue preparations and can be used as a nuclear marker in subcellular fractions. It produces a speckled pattern in normal and malignant cells and may be used to stain the nuclei of cells in fixed or frozen tissue sections. It can also be used with paraformaldehyde fixed frozen tissue or cell preparations.

Flow Cytometry, IF, IP, ICC

Flow Cytometry: 1-2 µg/million cells, IF: 1-2 µg/ml, IP: 1-2µg / 500µg proteins, ICC: 1-2 µg/ml, IHC-Fr: 1-2 µg/ml

Human, Non-Human Primates

This antibody does not cross react with Mouse, Rat, or Chicken.

Nuclei of human myeloid leukemia biopsy cells.

Rabbit

Monoclonal

235-1R

kappa

Unconjugated

Protein A/G chromatography.

200 µg/ml

70 kDa / 80 kDa

Liquid

Supplied in 10mM Phosphate Buffered Saline with 0.05% BSA and 0.05% Sodium Azide.

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

This monoclonal antibody is also available in a different formulation without BSA and Sodium Azide - Anti-Human Nuclear Antigen Antibody [235-1R] - BSA and Azide free (A278695).