Human Phosphoinositide-3-Kinase Catalytic Delta Polypeptide ELISA Kit (A5423)

Human Phosphoinositide-3-Kinase Catalytic Delta Polypeptide ELISA Kit

Sandwich (quantitative)

The microtiter plate provided in this kit has been pre-coated with an antibody specific to the index. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific to the index. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain the index, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of the index in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Microplate

Colorimetric

Tissue homogenates, cell lysates and other biological fluids.

0.312 - 20 ng/ml

1 - 4.5 Hours

Human

5-bisphosphate 3-kinase 110 kDa catalytic subunit delta, 5-bisphosphate 3-kinase catalytic subunit delta isoform, APDS, GRB1, IMD14, p110d, p110delta, p110dp85a, p85-ALPHA, Phosphatidylinositol 3 kinase catalytic delta polypeptide, Phosphatidylinositol 4 5 bisphosphate 3 kinase catalytic subunit delta isoform, Phosphatidylinositol 4,5 bisphosphate 3 kinase 110 kDa catalytic subunit delta, Phosphatidylinositol 4,5 bisphosphate 3 kinase, catalytic subunit delta, Phosphatidylinositol 45 bisphosphate 3 kinase catalytic subunit delta isoform, Phosphatidylinositol-4, Phosphoinositide 3 kinase B, Phosphoinositide 3 kinase C, Phosphoinositide 3 kinase, catalytic, delta polypeptide variant p37delta, PI 3 Kinase p110 delta, PI3 kinase p110 subunit delta, PI3-kinase subunit delta, PI3K, PI3K-delta, PI3Kdelta, Pik3cd, PIK3R1, PK3CD, PK3CD_HUMAN, PtdIns 3 kinase p110, PtdIns 3 kinase subunit p110 delta, PtdIns-3-kinase subunit delta, PtdIns-3-kinase subunit p110-delta