Principle of Assay
This Human PIK3Cß ELISA Kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of Human PIK3Cß in plasma, tissue homogenates and other biological fluids.
An antibody specific for PIK3Cß has been coated on a microplate. Standards and samples are pipetted into the wells and the PIK3Cß present in each sample is bound to the wells by the immobilised antibody. The wells are washed and Anti-PIK3Cß Antibody (Biotin) is added. After washing away the unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted into the wells. The wells are washed again, before a TMB substrate solution is added to the wells and colour develops in proportion to the amount of PIK3Cß bound. The Stop Solution changes the colour from blue to yellow, and the intensity of the colour is measured at 450 nm.
