Human Ribonucleotide Reductase M1 ELISA Kit (A6470)

Human Ribonucleotide Reductase M1 ELISA Kit

Sandwich (quantitative)

The microtiter plate provided in this kit has been pre-coated with an antibody specific to the index. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific to the index. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain the index, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of the index in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Microplate

Colorimetric

0.27 ng/ml

0.781 - 50 ng/ml

1 - 4.5 Hours

Human

R1, Ribonucleoside diphosphate reductase large subunit, Ribonucleoside diphosphate reductase M1 chain, Ribonucleoside diphosphate reductase subunit M1, Ribonucleoside reductase, large subunit, Ribonucleoside-diphosphate reductase large subunit, Ribonucleoside-diphosphate reductase subunit M1, Ribonucleotide reductase large chain, Ribonucleotide reductase large subunit, Ribonucleotide reductase M1 polypeptide, Ribonucleotide reductase R1 subunit, Ribonucleotide reductase, M1 subunit, RIR 1, RIR1, RIR1_HUMAN, RR 1, RR1, RRM 1, RRM1