Human RPE65 ELISA Kit (A77257)

Human RPE65 ELISA Kit

Sandwich (quantitative)

This Human RPE65 ELISA Kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of Human RPE65 in plasma, tissue homogenates and other biological fluids.

An antibody specific for RPE65 has been coated on a microplate. Standards and samples are pipetted into the wells and the RPE65 present in each sample is bound to the wells by the immobilised antibody. The wells are washed and Anti-RPE65 Antibody (Biotin) is added. After washing away the unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted into the wells. The wells are washed again, before a TMB substrate solution is added to the wells and colour develops in proportion to the amount of RPE65 bound. The Stop Solution changes the colour from blue to yellow, and the intensity of the colour is measured at 450 nm.

Pre-coated Microplate (12 x 8 well strips)

Colorimetric

Colorimetric Microplate Reader

Serum, plasma, tissue homogenates, and other biological fluids.

46.875 pg/ml

78.125-5000 pg/ml

4h 30m

Intra-assay CV: < 8%, Inter-assay CV: < 10%

Information online is representative. Refer to the Instructions for Use inside the kit.

Store at +4°C. Do not use past expiration date!

All-trans-retinyl-palmitate hydrolase, Lutein isomerase, Meso-zeaxanthin isomerase, Retinal pigment epithelium-specific 65 kDa protein, Retinoid isomerohydrolase, Retinol isomerase

Information online is representative. Always refer to the Product Manual inside the kit.