Human TIMP-1 ELISA Kit (A1527)

Human TIMP-1 ELISA Kit

Sandwich (quantitative)

Human TIMP-1 ELISA Kit is based on the quantitative sandwich enzyme-linked immunosorbent assay technique to measure concentration of human TIMP-1 in the samples. A monoclonal antibody specific for human TIMP-1 has been immobilized onto microwells. Standard or samples are pipetted into the wells, followed by the addition of biotin-linked detect antibody specific for TIMP-1, and TIMP-1 present is bound by the immobilized antibody and detect antibody following the first incubation. After removal of any unbound substances, streptavidin-HRP is added for a second incubation. After washing, substrate solution reacts with HRP and color develops in proportion to the amount of TIMP-1 bound by the immobilized antibody. The color development is stopped by addition of acid and the optical density value is measured by microplate reader.

Microplate

Colorimetric

Cell culture supernatant, serum and plasma.

31.25 - 2000 pg/ml

This kit can assay both natural and recombinant human TIMP-1.

Human

This assay has shown no significant cross-reactivity.

Store at 2 - 8°C.

Clgi, Collagenase inhibitor, Collagenase inhibitor, Human, EPA, EPO, Erythroid Potentiating Activity, Erythroid-potentiating activity, Fibroblast collagenase inhibitor, FLJ90373, HCI, Human Collagenase Inhibitor, Metalloproteinase inhibitor 1, Metalloproteinase inhibitor 1 precursor, OTTHUMP00000023214 , TIMP, TIMP 1, TIMP metallopeptidase inhibitor 1 , TIMP1, TIMP1 protein, TIMP1_HUMAN, Tissue Inhibitor of Metalloproteinase 1, Tissue inhibitor of metalloproteinases, Tissue inhibitor of metalloproteinases 1, Ttissue inhibitor of metalloproteinase 1 erythroid potentiating activity collagenase inhibitor