Human vWF ELISA Kit is based on the quantitative sandwich enzyme-linked immunosorbent assay technique to measure concentration of human vWF in the samples. A monoclonal antibody specific for human vWF has been immobilized onto microwells. Standard or samples are pipetted into the wells, and vWF present is bound by the immobilized antibody. After washing away any unbound substances, a biotin-linked monoclonal antibody specific for vWF is added to the wells. Following a wash to remove any unbound detect antibody, streptavidin-HRP is added. After washing, substrate solution reacts with HRP and color develops in proportion to the amount of vWF bound by the immobilized antibody. The color development is stopped by addition of acid and the optical density value is measured by microplate reader.
Platform
Microplate
Detection Type
Colorimetric
Sample Type
Cell culture supernatant, serum and plasma.
Sensitivity
0.47 pg/ml
Product Range
78.13 - 5000 pg/ml
Specificity
This kit can assay both natural and recombinant human vWF.
Reactivity
Human
Cross Reactivity
This assay has shown no significant cross-reactivity.
Storage
Store at 2 - 8°C.
Synonyms
Coagulation factor VIII , Coagulation factor VIII VWF, F8VWF, Factor VIII related antigen, von Willebrand antigen 2, von Willebrand antigen II, Von Willebrand disease, Von Willebrand Factor, VWD, VWF_HUMAN
Disclaimer
This product is for research use only. It is not intended for diagnostic or therapeutic use.
