Human Wingless Type MMTV Integration Site Family, Member 5B ELISA Kit
Assay Type
Sandwich (quantitative)
Principle of Assay
The microtiter plate provided in this kit has been pre-coated with an antibody specific to the index. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific to the index. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain the index, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of the index in the samples is then determined by comparing the O.D. of the samples to the standard curve.
Platform
Microplate
Detection Type
Colorimetric
Sample Type
Tissue homogenates, cell lysates and other biological fluids.
Sensitivity
0.53 ng/ml
Product Range
1.56 - 100 ng/ml
Assay Time
1 - 4.5 Hours
Reactivity
Human
Synonyms
MGC2648, Protein Wnt-5b, WNT 5B, WNT 5B protein, Wnt family member 5B, Wnt5b, WNT5B protein, WNT5B_HUMAN
Disclaimer
This product is for research use only. It is not intended for diagnostic or therapeutic use.
Figure 1: Standard Curve - Human Wingless Type MMTV Integration Site Family, Member 5B ELISA Kit (A6109)
Representative Standard Curve using Human WNT5B ELISA Kit (DL-WNT5B-Hu)
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