Anti-HuR / ELAVL1 Antibody [ARC0101] (A307808)

Name: Anti-HuR / ELAVL1 Antibody [ARC0101]
Brand: Antibodies.com
SKU: A307808
Price: 470.0 USD
Availability: InStock

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$470

Rabbit monoclonal [ARC0101] antibody to HuR / ELAVL1 for WB, IHC, ICC/IF and IP.

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Delivery

Freight Charges

$40 to United States

Lead Time

5-8 Business Days

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Knockout Validated Antibody

Specifications

Name

Anti-HuR / ELAVL1 Antibody [ARC0101]

Description

Rabbit monoclonal [ARC0101] antibody to HuR / ELAVL1.

Applications

WB, IHC, ICC/IF, IP

Dilutions

WB: 1:500-1:2,000, IHC: 1:50-1:200, ICC/IF: 1:50-1:200, IP: 1:500-1:1,000

Reactivity

Human, Mouse, Rat

Immunogen

A synthetic peptide corresponding to a sequence within amino acids 1-100 of human HuR/ELAVL1 (Q15717).

Sequence

MSNGYEDHMAEDCRGDIGRTNLIVNYLPQNMTQDELRSLFSSIGEVESAKLIRDKVAGHSLGYGFVNYVTAKDAERAINTLNGLRLQSKTIKVSYARPSS

Host

Rabbit

Clonality

Monoclonal

Clone ID

ARC0101

Isotype

IgG

Conjugate

Unconjugated

Purification

Affinity purification.

Molecular Weight

36 kDa

Product Form

Liquid

Formulation

Supplied in Phosphate Buffered Saline, pH 7.3, with 50% Glycerol, 0.05% BSA, and 0.02% Sodium Azide.

Storage

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

Synonyms

ELAV (embryonic lethal abnormal vision Drosophila) like 1, ELAV (embryonic lethal, abnormal vision, Drosophila) like 1 (Hu antigen R), ELAV like 1, ELAV like RNA binding protein 1, ELAV-like protein 1, ELAV1, ELAV1_HUMAN, Elavl1, Embryonic lethal abnormal vision drosophila homolog like 1, Hu Antigen R, Hu-antigen R, Hua, HuR, MelG

Isotype Controls

Suitable Secondaries

See all Anti-Rabbit IgG Secondaries →

Disclaimer

This product is for research use only. It is not intended for diagnostic or therapeutic use.

Scientific Validation Data (9)

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Figure 1: Western Blot - Anti-HuR / ELAVL1 Antibody [ARC0101] (A307808)

Western blot analysis of extracts from wild type (WT) and HuR/ELAVL1 knockout (KO) 293T cells, using Anti-HuR / ELAVL1 Antibody [ARC0101] (A307808) at 1:1,000 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 1s.

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Figure 2: Western Blot - Anti-HuR / ELAVL1 Antibody [ARC0101] (A307808)

Western blot analysis of extracts of various cell lines, using Anti-HuR / ELAVL1 Antibody [ARC0101] (A307808) at 1:1,000 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 1s.

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Figure 3: Immunohistochemistry - Anti-HuR / ELAVL1 Antibody [ARC0101] (A307808)

Immunohistochemistry analysis of paraffin-embedded rat liver using Anti-HuR / ELAVL1 Antibody [ARC0101] (A307808) at a dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.

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Figure 4: Immunohistochemistry - Anti-HuR / ELAVL1 Antibody [ARC0101] (A307808)

Immunohistochemistry analysis of paraffin-embedded human esophageal using Anti-HuR / ELAVL1 Antibody [ARC0101] (A307808) at a dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.