Anti-Iba1 Antibody [ARC51834 + ARC51835] (A307827)

Name: Anti-Iba1 Antibody [ARC51834 + ARC51835]
Brand: Antibodies.com
SKU: A307827
Price: 470.0 USD
Availability: InStock

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$470

Rabbit Oligoclonal [ARC51834 + ARC51835] antibody to Iba1 for WB, IHC and ICC/IF.

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Freight Charges

$40 to United States

Lead Time

5-8 Business Days

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Specifications

Name

Anti-Iba1 Antibody [ARC51834 + ARC51835]

Description

Rabbit Oligoclonal [ARC51834 + ARC51835] antibody to Iba1.

Applications

WB, IHC, ICC/IF

Dilutions

WB: 1:500-1:1,000, IHC: 1:500-1:1,000, ICC/IF: 1:50-1:200

Reactivity

Human, Mouse, Rat

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 1-147 of human AIF1/IBA1 Rabbit PolymAb® (NP_001614.3).

Sequence

MSQTRDLQGGKAFGLLKAQQEERLDEINKQFLDDPKYSSDEDLPSKLEGFKEKYMEFDLNGNGDIDIMSLKRMLEKLGVPKTHLELKKLIGEVSSGSGETFSYPDFLRMMLGKRSAILKMILMYEEKAREKEKPTGPPAKKAISELP

Host

Rabbit

Clonality

Oligoclonal

Clone ID

ARC51834 + ARC51835

Isotype

IgG

Conjugate

Unconjugated

Purification

Affinity purification.

Molecular Weight

17 kDa

Product Form

Liquid

Formulation

Supplied in Phosphate Buffered Saline, pH 7.3, with 50% Glycerol, 0.05% BSA, and 0.05% Proclin 300.

Storage

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

Synonyms

AIF 1, AIF-1, Aif1, AIF1 protein, AIF1_HUMAN, Allograft inflammatory factor 1, Allograft inflammatory factor 1 splice variant G, allograft inflammatory factor-1 splice variant Hara-1, balloon angioplasty responsive transcription, BART 1, G1, G1 putative splice variant of allograft inflamatory factor 1, IBA 1, interferon gamma responsive transcript, Interferon responsive transcript 1, interferon responsive transcript factor 1, Ionized calcium binding adapter molecule 1, ionized calcium-binding adapter molecule, Ionized calcium-binding adapter molecule 1, IRT 1, IRT1, Microglia response factor, MRF1, Protein g1

Isotype Controls

Suitable Secondaries

See all Anti-Rabbit IgG Secondaries →

Disclaimer

This product is for research use only. It is not intended for diagnostic or therapeutic use.

Scientific Validation Data (7)

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Figure 1: Western Blot - Anti-Iba1 Antibody [ARC51834 + ARC51835] (A307827)

Western blot analysis of various lysates, using Anti-Iba1 Antibody [ARC51834 + ARC51835] (A307827) at 1:1,000 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Enhanced Kit (RM00021). Exposure time: 120s.

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Figure 2: Immunohistochemistry - Anti-Iba1 Antibody [ARC51834 + ARC51835] (A307827)

Immunohistochemistry analysis of paraffin-embedded human brain tissue using Anti-Iba1 Antibody [ARC51834 + ARC51835] (A307827) at a dilution of 1:800 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.

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Figure 3: Immunohistochemistry - Anti-Iba1 Antibody [ARC51834 + ARC51835] (A307827)

Immunohistochemistry analysis of paraffin-embedded human lung using Anti-Iba1 Antibody [ARC51834 + ARC51835] (A307827) at a dilution of 1:800 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.

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Figure 4: Immunohistochemistry - Anti-Iba1 Antibody [ARC51834 + ARC51835] (A307827)

Immunohistochemistry analysis of paraffin-embedded human tonsil using Anti-Iba1 Antibody [ARC51834 + ARC51835] (A307827) at a dilution of 1:800 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.